Agriculture and Agri-Food Canada, Dairy and Swine Research and Development Centre, Sherbrooke, Quebec, Canada.
J Dairy Sci. 2013 Aug;96(8):5151-60. doi: 10.3168/jds.2012-6392. Epub 2013 Jun 13.
Different protein sources, such as canola meal (CM) or dried distillers grains (DDG), are currently used in dairy rations to replace soybean meal (SBM). However, little data exists comparing their rumen degradation in a single study. Therefore, the objective of this study was to compare the ruminal degradation of dry matter (DM), crude protein (CP), and AA of SBM, CM, high-protein corn DDG (HPDDG), and wheat DDG plus solubles (WDDGS). In situ studies were conducted with 4 rumen-fistulated lactating Holstein cows fed a diet containing 38% grass hay and 62% corn-based concentrate. Each protein source was incubated in the rumen of each cow in nylon bags for 0, 2, 4, 8, 16, 24, and 48 h to determine DM and CP rumen degradation kinetics, whereas additional bags were also incubated for 16 h to evaluate AA ruminal disappearance. Rumen DM and CP degradability was calculated from rumen-undegraded residues corrected or not for small particle loss. Data were fitted to an exponential model to estimate degradation parameters and effective degradability (ED) was calculated with a passage rate of 0.074 h(-1). The WDDGS and SBM had higher uncorrected ED (DM=75.0 and 72.6%; CP=84.8 and 66.0%, respectively) than CM and HPDDG (DM=57.2 and 55.5%; CP=59.3 and 48.2%, respectively), due to higher soluble fraction in WDDGS and a combination of higher potentially degradable fraction and rate of degradation in SBM. Correction for small particle loss from bags, higher for WDDGS than for the other protein sources, decreased estimated ED but did not alter feed ranking. The ruminal disappearance of AA after 16 h of incubation reflected the overall pattern of CP degradation between protein supplements, but the ruminal disappearance of individual AA differed between protein supplements. Overall, these results indicate that, in the current study, (1) SBM and WDDGS were more degradable in the rumen than CM and HPDDG, and (2) that small particle loss correction is relevant but does not alter this ranking.
不同的蛋白质来源,如菜粕(CM)或干酒糟及其可溶物(DDG),目前被用于奶牛日粮中以替代豆粕(SBM)。然而,在单一研究中比较它们在瘤胃中的降解情况的数据很少。因此,本研究的目的是比较 SBM、CM、高蛋白玉米 DDG(HPDDG)和小麦 DDG 加可溶物(WDDGS)的干物质(DM)、粗蛋白(CP)和 AA 在瘤胃中的降解情况。采用尼龙袋法在 4 头奶牛的瘤胃中进行了原位研究,这些奶牛的日粮中含有 38%的干草和 62%的玉米基浓缩饲料。每个蛋白质源在每个奶牛的瘤胃中孵育 0、2、4、8、16、24 和 48 小时,以确定 DM 和 CP 瘤胃降解动力学,而其他袋子也孵育 16 小时以评估 AA 在瘤胃中的消失情况。通过校正或不校正小颗粒损失来计算瘤胃未降解残留物中的瘤胃 DM 和 CP 降解率。数据用指数模型拟合来估计降解参数,并用 0.074 h(-1)的通过速率计算有效降解率(ED)。WDDGS 和 SBM 的未校正 ED(DM=75.0 和 72.6%;CP=84.8 和 66.0%)高于 CM 和 HPDDG(DM=57.2 和 55.5%;CP=59.3 和 48.2%),这是因为 WDDGS 中的可溶性部分较高,而 SBM 中潜在可降解部分和降解速率较高。校正袋子中小颗粒损失后,WDDGS 比其他蛋白质来源的校正值更高,降低了估计的 ED,但没有改变饲料的排序。孵育 16 小时后 AA 的瘤胃消失反映了蛋白质补充物之间 CP 降解的总体模式,但单个 AA 的瘤胃消失在蛋白质补充物之间存在差异。总的来说,这些结果表明,在本研究中,(1)SBM 和 WDDGS 在瘤胃中的降解速度快于 CM 和 HPDDG,(2)小颗粒损失校正虽然相关但不改变这种排序。