Wang Jie, Guo Liqiong, Lin Junfang
Department of Bioengineering, College of Food Science, and Institute of Biomass Research, South China Agricultural University, Guangzhou, China.
J Agric Food Chem. 2009 Mar 25;57(6):2392-6. doi: 10.1021/jf803363g.
Transgenic Volvariella volvacea strains, which are tolerant to cold stress, were generated by the stable insertion of antifreeze protein gene isolated from budworm into the genome of a conventional variety V23 of V. volvacea. As a part of the safety assessment program, transgenic V. volvacea strains were compared to a nontransgenic near-isogenic V. volvacea strain V23 grown contemporaneously by applying the principle of substantial equivalence. Compositional analyses were conducted by measuring proximates, amino acids, fatty acids, minerals, vitamins, 5'-nucleotides, nucleic acid, and antinutrients such as tannin and cyanide in V. volvacea strains harvested at egg-shaped stage. Results of the comparisons indicate that these transgenic strains are compositionally equivalent to the conventional control.
通过将从芽虫中分离出的抗冻蛋白基因稳定插入草菇常规品种V23的基因组中，培育出了耐冷胁迫的转基因草菇菌株。作为安全评估计划的一部分，根据实质等同原则，将转基因草菇菌株与同期种植的非转基因近等基因草菇菌株V23进行了比较。通过测量卵形期收获的草菇菌株中的粗养分、氨基酸、脂肪酸、矿物质、维生素、5'-核苷酸、核酸以及单宁和氰化物等抗营养物质进行了成分分析。比较结果表明，这些转基因菌株在成分上与传统对照相当。
