McDermott Ann
Department of Chemistry, Columbia University, New York, NY 10027, USA.
Annu Rev Biophys. 2009;38:385-403. doi: 10.1146/annurev.biophys.050708.133719.
Membrane proteins remain difficult to study by traditional methods. Magic angle spinning solid-state NMR (MAS SSNMR) methods present an important approach for studying membrane proteins of moderate size. Emerging MAS SSNMR methods are based on extensive assignments of the nuclei as a basis for structure determination and characterization of function. These methods have already been used to characterize fibrils and globular proteins and are being increasingly used to study membrane proteins embedded in lipids. This review highlights recent applications to intrinsic membrane proteins and summarizes recent technical advances that will enable these methods to be utilized for more complex membrane protein systems in the near future.
膜蛋白一直难以用传统方法进行研究。魔角旋转固态核磁共振（MAS SSNMR）方法是研究中等大小膜蛋白的一种重要方法。新兴的MAS SSNMR方法基于对原子核的广泛归属，以此作为结构测定和功能表征的基础。这些方法已被用于表征纤维状蛋白和球状蛋白，并且越来越多地用于研究嵌入脂质中的膜蛋白。本综述重点介绍了其在内在膜蛋白方面的最新应用，并总结了近期的技术进展，这些进展将使这些方法在不久的将来能够用于更复杂的膜蛋白系统。
