Gornet M F, Randolph M A, Schofield B H, Yaremchuk M J, Weiland A J
Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, Md.
Plast Reconstr Surg. 1991 Nov;88(5):860-8. doi: 10.1097/00006534-199111000-00019.
This investigation evaluated ultrastructural changes during the earliest phase of immunologic rejection of vascularized bone allografts in a genetically defined rat model. These results were correlated with the cell-mediated and humoral immunologic responses during this time period. Employing a model for heterotopic allograft transplantation, 33 rats divided into four categories were evaluated. Group I consisted of ungrafted (naive) Lewis and Brown Norway rats; group II consisted of Lewis-to-Lewis vascularized bone isografts; group III consisted of Lewis-to-Brown Norway vascularized bone allografts; and group IV consisted of Lewis-to-Brown Norway vascularized bone allografts in rats receiving cyclosporine (10 mg/kg/day). Experimental animals were sacrificed at 3, 5, and 7 days. Immunologic analysis was performed using a cell-mediated lymphocytotoxicity assay and a complement-dependent cytotoxic antibody assay. The results of this study show that rejection of vascularized bone allografts appears as early as 3 days postoperatively, with osteocytes and vascular endothelium being the first elements affected. This early rejection is probably a manifestation of the humoral response. All changes secondary to rejection were arrested by cyclosporine.
本研究在一个基因明确的大鼠模型中，评估了血管化同种异体骨移植免疫排斥反应最早阶段的超微结构变化。这些结果与该时间段内的细胞介导免疫反应和体液免疫反应相关。采用异位同种异体移植模型，对分为四类的33只大鼠进行了评估。第一组由未移植（未处理）的Lewis大鼠和Brown Norway大鼠组成；第二组由Lewis大鼠到Lewis大鼠的血管化骨同基因移植组成；第三组由Lewis大鼠到Brown Norway大鼠的血管化骨同种异体移植组成；第四组由接受环孢素（10毫克/千克/天）的Lewis大鼠到Brown Norway大鼠的血管化骨同种异体移植组成。实验动物在术后3天、5天和7天处死。使用细胞介导的淋巴细胞毒性试验和补体依赖性细胞毒性抗体试验进行免疫分析。本研究结果表明，血管化同种异体骨移植的排斥反应最早在术后3天出现，骨细胞和血管内皮是首先受到影响的成分。这种早期排斥反应可能是体液反应的一种表现。环孢素阻止了排斥反应继发的所有变化。
