酵母交配型基因座处DNA双链断裂形成过程中的染色质重塑分析。
Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus.
作者信息
Tsukuda Toyoko, Trujillo Kelly M, Martini Emmanuelle, Osley Mary Ann
机构信息
Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, United States.
出版信息
Methods. 2009 May;48(1):40-5. doi: 10.1016/j.ymeth.2009.02.007. Epub 2009 Feb 24.
Abstract
DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.
摘要
DNA修复发生在染色质环境中，现在核小体重塑被认为是一种重要的调控特征，因为它能使修复因子接近受损位点。酵母交配型基因座（MAT）已成为研究在明确的DNA双链断裂（DSB）处染色质重塑作用的优秀模型。我们讨论了在DSB形成后研究MAT基因座处核小体动态变化以及DSB修复因子招募情况的方法。
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