Beguelini Mateus R, Moreira Paula R L, Faria Karina C, Marchesin Sandra R C, Morielle-Versute Eliana
Department of Zoology and Botany, São Paulo State University-UNESP/IBILCE, São José do Rio Preto, São Paulo, 15054-000, Brazil.
J Morphol. 2009 Aug;270(8):943-53. doi: 10.1002/jmor.10731.
We know little about the process of spermatogenesis in bats, a great and diverse clade of mammals that presents different reproductive strategies. In the present study, spermatogenesis in six species of Neotropical bats was investigated by light microscopy. On the basis of chromatin condensation, nuclear morphology, relative position to the basal membrane and formation of the flagellum, three types of spermatogonia were recognized: dark type A (A(d)), pale type A (A(p)), and type B; the development of spermatids was divided into seven steps. With the exception of Myotis nigricans, the seminiferous epithelium cycle of the other five species studied was similar to those of other mammals, showing gradual stages by the tubular morphology method. Asynchrony was observed in the seminiferous epithelium cycle of M. nigricans, shown by overlapping stages and undefined cycles. The frequencies found in the three phases of the cycle were variable with the greatest frequency occurring in the postmeiotic phase (>50%) and the least in the meiotic phase (<10%). The similarities observed in the five species of Phyllostomidae appeared to be related to their phylogenetic relationship and shorter divergence times, whereas the differences in M. nigricans appeared to be related to its greater phylogenetic distance because the Vespertilionidae family diverged earlier.
我们对蝙蝠的精子发生过程知之甚少，蝙蝠是一个种类繁多的哺乳动物类群，具有不同的繁殖策略。在本研究中，通过光学显微镜对六种新热带蝙蝠的精子发生进行了研究。根据染色质浓缩、核形态、与基底膜的相对位置以及鞭毛的形成，识别出三种类型的精原细胞：暗A型（A(d)）、淡A型（A(p)）和B型；精子细胞的发育分为七个步骤。除了黑鼠耳蝠外，所研究的其他五个物种的生精上皮周期与其他哺乳动物相似，通过管状形态学方法呈现出渐进阶段。在黑鼠耳蝠的生精上皮周期中观察到不同步现象，表现为阶段重叠和周期不明确。在周期的三个阶段中发现的频率各不相同，减数分裂后阶段的频率最高（>50%），减数分裂阶段的频率最低（<10%）。叶口蝠科五个物种中观察到的相似性似乎与其系统发育关系和较短分化时间有关，而黑鼠耳蝠的差异似乎与其更大的系统发育距离有关，因为蝙蝠科分化较早。
