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[利用原子吸收分光光度计分析盐胁迫对披碱草属植物矿质元素吸收与积累的影响]

[Analysis of effects of salt stress on absorption and accumulation of mineral elements in Elymus spp. using atomic absorption spectrophotometer].

作者信息

Jia Ya-xiong, Sun Lei, He Feng, Wan Li-qiang, Yuan Qing-hua, Li Xiang-lin

机构信息

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2008 Dec;28(12):2984-8.

Abstract

Salinization contributes significantly to soil degradation and the growth and survival of plants. A high level of salts imposes both ionic and osmotic stresses on plants, resulting in an excessive accumulation of sodium (Na) in plant tissues. Na toxicity disrupts the uptake of soil nutrients. Plant uptake and absorption of macro-elements under salt stress have been studied in plants, but there is little literature addressing the effect of salt stress on plant accumulation and absorption of micro-elements. Species in Elymus genus are among the most important forage plants on high-salinity soils in China An experiment was conducted to study the effect of salt stress on accumulation and absorption of both macro- and micro-elements by wild plants of Elymus genus. Plant samples taken from two populations with different salt tolerance were tested and the level of 4 macro-elements, namely Na, K, Ca and Mg, and 4 micro-elements, namely Cu, Fe, Mn, Zn was determined using atomic absorption spectrophotometer. The relationship between the selection of elements in the process of absorption and accumulation and salt tolerance was also analyzed. The results showed that the level of Na in root and leaf tissues increased with increasing salt stress. The level of Na in leaf tissue of plants with high salt tolerance (HS) was significantly higher than that in plants with low salt tolerance (P<0.05). The level of K and Ca decreased in response to increasing salt stress, while that in HS was higher than in LS. The level of Fe and Zn in the tissues of both roots and leaves increased. No significant difference was detected between HS and LS samples in the level of Cu in root tissues, while that of Cu in leaf tissue of both samples increased. The level of Mn decreased with increasing salt stress, but was higher in HS than in LS. Fe and Zn in roots and leaves of HS were lower than in those of LS.

摘要

土壤盐渍化是导致土壤退化以及影响植物生长和存活的重要因素。高盐水平会给植物带来离子胁迫和渗透胁迫,导致植物组织中钠(Na)过量积累。钠毒会干扰植物对土壤养分的吸收。虽然已有研究关注盐胁迫下植物对大量元素的吸收情况,但关于盐胁迫对植物微量元素积累和吸收影响的文献较少。在中国,披碱草属植物是高盐碱土壤上最重要的饲草植物之一。本研究通过实验,探究盐胁迫对披碱草属野生植物大量元素和微量元素积累与吸收的影响。对采自两个耐盐性不同种群的植物样本进行测试,使用原子吸收分光光度计测定了4种大量元素(Na、K、Ca和Mg)和4种微量元素(Cu、Fe、Mn、Zn)的含量,并分析了植物吸收和积累过程中元素选择与耐盐性之间的关系。结果表明,随着盐胁迫加剧,根和叶组织中的Na含量增加。高耐盐性(HS)植物叶片组织中的Na含量显著高于低耐盐性植物(P<0.05)。随着盐胁迫增加,K和Ca含量下降,但HS中的含量高于低耐盐性植物(LS)。根和叶组织中的Fe和Zn含量均增加。根组织中Cu含量在HS和LS样本之间无显著差异,但两个样本叶片组织中的Cu含量均增加。随着盐胁迫增加,Mn含量下降,但HS中的含量高于LS。HS根和叶中的Fe和Zn含量低于LS。

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