Kivipelto L
Department of Anatomy, University of Helsinki, Finland.
Regul Pept. 1991 Jul 9;34(3):211-24. doi: 10.1016/0167-0115(91)90180-o.
The octapeptide FLFQPQRF-NH2 or neuropeptide FF ('F8Famide'; FMRFamide-like peptide'; 'morphine-modulating peptide') has been isolated from the bovine brain. In this study, the ultrastructural localization of neuropeptide FF-like immunoreactivity was examined with pre-embedding immuno-electron microscopy in the nucleus of the solitary tract and in the posterior lobe of the pituitary gland of an adult rat. Neuropeptide FF-like immunoreactivity was detected only in neuronal structures of the medial and commissural nuclei of the solitary tract and in the neurohypophysis. In the medulla, the peroxidase-antiperoxidase reaction product was localized in large (100 nm) dense-cored vesicles and in the cytoplasm of the neuronal perikarya, dendrites and axon terminals. In the labeled terminals, small (50 nm) clear vesicles rimmed with the peroxidase-antiperoxidase reaction product were seen. Synaptic contacts of labeled perikarya and dendrites with unlabeled axon terminals were observed. Labeled axon terminals formed contacts with unlabeled dendrites and perikarya. In the posterior lobe of the pituitary gland, neuropeptide FF-like immunoreactivity was localized in nerve terminals frequently associated with blood vessels. The results suggest that neuropeptide FF-like peptides are localized exclusively in neuronal structures and that they are synthesized in cell somata and released from axon terminals. In the brain, neuropeptide FF-like peptides may act as neuromodulators involved in the regulation of autonomic functions. The localization of neuropeptide FF-like immunoreactivity in the neurohypophysis suggests endocrine regulatory functions of these peptides.
八肽FLFQPQRF-NH2或神经肽FF（“F8F酰胺”；“FMRF酰胺样肽”；“吗啡调节肽”）已从牛脑中分离出来。在本研究中，采用包埋前免疫电子显微镜技术，对成年大鼠孤束核和垂体后叶中神经肽FF样免疫反应性的超微结构定位进行了检测。仅在孤束核内侧和连合核的神经元结构以及神经垂体中检测到神经肽FF样免疫反应性。在延髓中，过氧化物酶-抗过氧化物酶反应产物定位于大（100 nm）的致密核心小泡以及神经元胞体、树突和轴突终末的细胞质中。在标记的终末中，可见边缘带有过氧化物酶-抗过氧化物酶反应产物的小（50 nm）清亮小泡。观察到标记的胞体和树突与未标记的轴突终末之间的突触联系。标记的轴突终末与未标记的树突和胞体形成联系。在垂体后叶中，神经肽FF样免疫反应性定位于经常与血管相关的神经终末。结果表明，神经肽FF样肽仅定位于神经元结构中，它们在细胞体中合成并从轴突终末释放。在脑中，神经肽FF样肽可能作为神经调质参与自主功能的调节。神经肽FF样免疫反应性在神经垂体中的定位提示了这些肽的内分泌调节功能。
