Fried T A, Hishida A, Ayon M A, Barnes J L, Stein J H
Department of Medicine, University of Texas Health Science Center, San Antonio 78284-7882.
Ren Fail. 1991;13(1):5-13. doi: 10.3109/08860229109022140.
The effect of a 4.5 mL/h Ringer infusion on the recovery from a unilateral 40-min renal artery occlusion was investigated in Sprague-Dawley rats. The inulin clearance measured in the experimental kidney 24 and 48 h after the insult in control animals that did not receive the Ringer infusion was 0.14 +/- 0.10 (mean +/- SE) and 0.11 +/- 0.05 mL/min, respectively. In animals that received 24 h of Ringer infusion begun at the time of the renal artery occlusion the inulin clearance was 0.81 +/- 0.07 mL/min, a value significantly higher than either of the control groups (p less than .05). If, however, the Ringer infusion was stopped at 24 h and the inulin clearance measured at 48 h, it had decreased significantly (0.27 +/- 0.09 mL/min) and was no longer greater than the control groups. Similarly, if the infusion was continued for 48 h there was no longer a significant difference between the inulin clearance (0.37 +/- 0.11), when compared with 48 h of no infusion (0.11 +/- 0.05). The histology of the different groups corresponded with the functional data. We conclude that 24 h of Ringer infusion leads to functional and histological protection when measured at 24 h; however, if measured at 48 h, protection is no longer evident. These studies suggest that caution should be exercised in extrapolating from the results of protective maneuvers in ischemic acute renal failure investigated by short-term studies.
在斯普拉格-道利大鼠中，研究了以4.5 mL/h的速度输注林格液对单侧肾动脉闭塞40分钟后恢复情况的影响。在未接受林格液输注的对照动物中，损伤后24小时和48小时在实验肾中测得的菊粉清除率分别为0.14±0.10（平均值±标准误）和0.11±0.05 mL/分钟。在肾动脉闭塞时开始接受24小时林格液输注的动物中，菊粉清除率为0.81±0.07 mL/分钟，该值显著高于任何一个对照组（p<0.05）。然而，如果在24小时时停止林格液输注，并在48小时时测量菊粉清除率，则其显著下降（0.27±0.09 mL/分钟），且不再高于对照组。同样，如果输注持续48小时，与未输注48小时（0.11±0.05）相比，菊粉清除率（0.37±0.11）之间不再有显著差异。不同组的组织学与功能数据相符。我们得出结论，在24小时测量时，24小时的林格液输注可导致功能和组织学保护；然而，在48小时测量时，保护作用不再明显。这些研究表明，在从短期研究中缺血性急性肾衰竭的保护性操作结果进行外推时应谨慎。
