Goto M, Abe H, Ito-Sasaki T, Goto M, Inagaki A, Ogawa N, Fujimori K, Kurokawa Y, Matsue T, Satomi S
Tohoku University International Advanced Research and Education Organization, Tohoku University, Sendai, Japan.
Transplant Proc. 2009 Jan-Feb;41(1):311-3. doi: 10.1016/j.transproceed.2008.10.075.
The current methods for evaluating islet potency are not useful in clinical transplantation. Therefore, we need reliable, rapid methods enabling accurate prediction of islet quality.
We evaluated respiratory activity using scanning electrochemical microscopy (SECM), glucose-stimulated respiratory activity, glucose-stimulated insulin release, ADP/ATP assays, insulin/DNA levels, and Trypan blue exclusion tests as predictive methods for the ability of isolated rat islets to cure syngeneic diabetic rats.
Although glucose-stimulated respiratory activity, basal respiratory activity, ADP/ATP ratio, and glucose-stimulated insulin release were significantly correlated with the outcome of transplantation into diabetic rats, there was no correlation between outcomes, insulin/DNA ratios, and Trypan blue exclusion tests. The glucose-stimulated respiratory activity in islet preparations that could cure diabetic rats was significantly greater than those unable to cure diabetes. Rat islets with >1.5-fold glucose-stimulated respiratory activity consistently cured diabetic rats, whereas those with a value <1.5 hardly cured any rats.
Measurement of the glucose-stimulated respiratory activity using SECM technique is a novel method that may be useful as a rapid, potent predictor of the outcome of clinical islet transplantation.
目前评估胰岛效力的方法在临床移植中并无用处。因此，我们需要可靠、快速的方法来准确预测胰岛质量。
我们使用扫描电化学显微镜（SECM）评估呼吸活性、葡萄糖刺激的呼吸活性、葡萄糖刺激的胰岛素释放、ADP/ATP检测、胰岛素/DNA水平以及台盼蓝排斥试验，作为分离的大鼠胰岛治愈同基因糖尿病大鼠能力的预测方法。
尽管葡萄糖刺激的呼吸活性、基础呼吸活性、ADP/ATP比值以及葡萄糖刺激的胰岛素释放与移植到糖尿病大鼠体内的结果显著相关，但结果、胰岛素/DNA比值和台盼蓝排斥试验之间并无相关性。能够治愈糖尿病大鼠的胰岛制剂中的葡萄糖刺激呼吸活性显著高于无法治愈糖尿病的胰岛制剂。葡萄糖刺激呼吸活性大于1.5倍的大鼠胰岛始终能治愈糖尿病大鼠，而该值小于1.5的胰岛几乎无法治愈任何大鼠。
使用SECM技术测量葡萄糖刺激的呼吸活性是一种新方法，可能作为临床胰岛移植结果的快速、有效预测指标。
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