Takahashi H, Goto M, Ogawa N, Saito Y, Fujimori K, Kurokawa Y, Doi H, Satomi S
Tohoku University International Advanced Research and Education Organization, Sendai, Japan.
Transplant Proc. 2009 Jan-Feb;41(1):350-1. doi: 10.1016/j.transproceed.2008.08.143.
It has recently been reported that the outcomes of islet transplantation with short periods of culture are comparable with those of freshly isolated islets. To clarify the influence of culture, fresh islets were compared with cultured islets in terms of quality.
The quality of freshly isolated islets was compared with that of cultured islets with CMRL 1066 including 10% allogeneic serum, CMRL 1066 including 0.5% human serum albumin, or Miami medium. We evaluated static glucose stimulation tests, insulin/DNA contents, ADP/ATP ratios, and an intraportal transplantation model into syngeneic diabetic rats. The expression of inflammatory mediators in the islets was examined using Western blotting for tissue factor (TF), which is the initiator of detrimental instant, blood-mediated, inflammatory reactions (IBMIR).
Although the survival rate was similar in all groups, the stimulation index upon glucose challenge and the insulin/DNA ratio were significantly higher among fresh islets. Most importantly, the expression of TF on islets was significantly lower in fresh islets, suggesting that culture enhanced TF-dependent IBMIR after transplantation. In an in vivo transplantation model, the curative rate and insulin production by the recipient liver was considerably greater in the fresh islet group.
Isolated islets without prior culture showed results superior to cultured islets.
最近有报道称，短期培养的胰岛移植结果与新鲜分离的胰岛相当。为了阐明培养的影响，我们从质量方面对新鲜胰岛和培养胰岛进行了比较。
将新鲜分离的胰岛的质量与用含10%同种异体血清的CMRL 1066、含0.5%人血清白蛋白的CMRL 1066或迈阿密培养基培养的胰岛的质量进行比较。我们评估了静态葡萄糖刺激试验、胰岛素/DNA含量、ADP/ATP比率以及将其移植到同基因糖尿病大鼠门静脉内的模型。使用针对组织因子（TF）的蛋白质印迹法检测胰岛中炎症介质的表达，TF是有害的即时血液介导炎症反应（IBMIR）的启动因子。
尽管所有组的存活率相似，但新鲜胰岛在葡萄糖刺激后的刺激指数和胰岛素/DNA比率显著更高。最重要的是，新鲜胰岛中TF在胰岛上的表达显著更低，这表明培养会增强移植后依赖TF的IBMIR。在体内移植模型中，新鲜胰岛组的治愈率和受体肝脏的胰岛素产生量明显更高。
未经预先培养的分离胰岛显示出优于培养胰岛的结果。
