家蚕核型多角体病毒ORF41（Bm41）参与芽生型病毒（BV）产生和包涵体衍生病毒（ODV）包膜形成。
Involvement of Bombyx mori nucleopolyhedrovirus ORF41 (Bm41) in BV production and ODV envelopment.
作者信息
Tian Cai-Hong, Zhao Jin-Fang, Xu Yi-Peng, Xue Jian, Zhang Bao-Qin, Cui Ying-Jun, Zhang Min-Juan, Bao Yan-Yuan, Zhang Chuan-Xi
机构信息
Institute of Insect Science, and Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Zhejiang University, Hangzhou 310029, China.
出版信息
Virology. 2009 Apr 25;387(1):184-92. doi: 10.1016/j.virol.2009.02.011. Epub 2009 Feb 27.
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF41 (Bm41), homologous to Ac52, is a gene present in most lepidopteran nucleopolyhedroviruses. Bm41 transcripts and encoded protein in BmNPV-infected cells can be detected from 3 and 6 h post-infection, respectively. Immunoassays have shown that Bm41 is not a viral structural protein and is detected in both the nuclei and cytoplasm of infected cells. A Bm41-disrupted virus (vBm(De)) and a repaired virus (vBm(Re)) were generated to investigate the function of Bm41. The results showed that Bm41 was essential for viral replication, and the disruption of Bm41 resulted in a much lower viral titer. Transmission electron microscopy revealed that disruption of Bm41 affected normal nucleocapsid envelopment and polyhedra formation in the nucleus. The disruption of Bm41 might severely affect odv-ec27 and polyhedrin expression. The disrupted virus reduced BmNPV infectivity in an LD(50) bioassay and took 18-23 h longer to kill larvae than wild-type virus in an LT(50) bioassay.
家蚕核型多角体病毒（BmNPV）的ORF41（Bm41）与Ac52同源，是大多数鳞翅目核型多角体病毒中存在的一个基因。在感染BmNPV的细胞中，分别在感染后3小时和6小时可以检测到Bm41转录本和编码蛋白。免疫分析表明，Bm41不是病毒结构蛋白，在感染细胞的细胞核和细胞质中均能检测到。构建了一个Bm41缺失病毒（vBm(De)）和一个修复病毒（vBm(Re)）来研究Bm41的功能。结果表明，Bm41对病毒复制至关重要，Bm41的缺失导致病毒滴度大幅降低。透射电子显微镜显示，Bm41的缺失影响了细胞核中正常核衣壳的包裹和多角体的形成。Bm41的缺失可能严重影响odv-ec27和多角体蛋白的表达。在LD(50)生物测定中，缺失病毒降低了BmNPV的感染力，在LT(50)生物测定中，杀死幼虫的时间比野生型病毒长18 - 23小时。
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