Institute of Biophysics, v.v.i., Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Physiol Res. 2010;59(1):133-137. doi: 10.33549/physiolres.931723. Epub 2009 Feb 27.
Four mouse bone marrow or thymus cell populations, namely granulopoietic/monocytopoietic, erythropoietic, B-lymphopoietic, and T-lymphopoietic precursor cells have been assayed by RT-PCR technique for the presence and relative amounts of adenosine A(1), A(2a), A(2b), and A(3) receptor mRNA. It has been found that (i) all four populations studied express all four adenosine receptor subtypes, (ii) the A(1), receptor is the least expressed in all populations studied, (iii) the A(3) receptor is markedly expressed in the populations of granulopoietic/monocytopoietic and erythropoietic cells, (iv) the A(2a) receptor is markedly expressed in the populations of B-lymphopoietic and T-lymphopoietic cells, and v) the A(2b) receptor does not predominate in any of the precursor cells studied. Our data offer a new possibility for the assessment of the readiness of these cells to respond, by receptor-mediated mechanisms, to adenosine or its analogs present in the tissues as a result of endogenous processes and/or following their administration.
四种小鼠骨髓或胸腺细胞群体，即粒细胞/单核细胞生成、红细胞生成、B 淋巴细胞生成和 T 淋巴细胞生成前体细胞，已通过 RT-PCR 技术检测到腺苷 A(1)、A(2a)、A(2b)和 A(3)受体 mRNA 的存在和相对量。研究发现：（i）所有四种研究的细胞群体都表达四种腺苷受体亚型；（ii）A(1)受体在所有研究的细胞群体中表达最少；（iii）A(3)受体在粒细胞/单核细胞生成和红细胞生成细胞群体中显著表达；（iv）A(2a)受体在 B 淋巴细胞生成和 T 淋巴细胞生成细胞群体中显著表达；（v）A(2b)受体在研究的任何前体细胞中都不占主导地位。我们的数据为评估这些细胞通过受体介导的机制对组织中存在的腺苷或其类似物的反应准备情况提供了新的可能性，这些反应是由于内源性过程和/或在其给药后引起的。
