Suppr超能文献

腺苷 A1 和 A3 受体的药理学激活不会调节小鼠造血干细胞和多能祖细胞的长期或短期再生能力。

The pharmacological activation of adenosine A1 and A 3 receptors does not modulate the long- or short-term repopulating ability of hematopoietic stem and multipotent progenitor cells in mice.

机构信息

Laboratory of Experimental Hematology, Institute of Biophysics, vvi, Academy of Sciences of the Czech Republic, Brno, Czech Republic.

出版信息

Purinergic Signal. 2013 Jun;9(2):207-14. doi: 10.1007/s11302-012-9340-5. Epub 2012 Dec 15.

Abstract

This study continues our earlier findings on the hematopoiesis-modulating effects of adenosine A1 and A3 receptor agonists that were performed on committed hematopoietic progenitor and precursor cell populations. In the earlier experiments, N (6)-cyclopentyladenosine (CPA), an adenosine A1 receptor agonist, was found to inhibit proliferation in the above-mentioned hematopoietic cell systems, whereas N (6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA), an adenosine A3 receptor agonist, was found to stimulate it. The topic of this study was to evaluate the possibility that the above-mentioned adenosine receptor agonists modulate the behavior of early hematopoietic progenitor cells and hematopoietic stem cells. Flow cytometric analysis of hematopoietic stem cells in mice was employed, as well as a functional test of hematopoietic stem and progenitor cells (HSPCs). These techniques enabled us to study the effect of the agonists on both short-term repopulating ability and long-term repopulating ability, representing multipotent progenitors and hematopoietic stem cells, respectively. In a series of studies, we did not find any significant effect of adenosine agonists on HSPCs in terms of their numbers, proliferation, or functional activity. Thus, it can be concluded that CPA and IB-MECA do not significantly influence the primitive hematopoietic stem and progenitor cell pool and that the hematopoiesis-modulating action of these adenosine receptor agonists is restricted to more mature compartments of hematopoietic progenitor and precursor cells.

摘要

本研究延续了我们之前关于腺苷 A1 和 A3 受体激动剂对造血作用的研究结果,这些研究是在定向造血祖细胞和前体细胞群上进行的。在之前的实验中,发现腺苷 A1 受体激动剂 N(6)-环戊基腺苷(CPA)抑制上述造血细胞系统的增殖,而腺苷 A3 受体激动剂 N(6)-(3-碘苄基)腺苷-5'-N-甲基尿苷酰胺(IB-MECA)刺激其增殖。本研究的主题是评估上述腺苷受体激动剂是否有可能调节早期造血祖细胞和造血干细胞的行为。我们采用了流式细胞术分析小鼠造血干细胞,并进行了造血干细胞和祖细胞(HSPCs)的功能测试。这些技术使我们能够研究激动剂对短期再殖能力和长期再殖能力的影响,分别代表多能祖细胞和造血干细胞。在一系列研究中,我们没有发现腺苷激动剂对 HSPCs 的数量、增殖或功能活性有任何显著影响。因此,可以得出结论,CPA 和 IB-MECA 对原始造血干细胞和祖细胞池没有显著影响,这些腺苷受体激动剂对造血的调节作用仅限于造血祖细胞和前体细胞的更成熟部分。

相似文献

2
Homeostatic action of adenosine A3 and A1 receptor agonists on proliferation of hematopoietic precursor cells.
Exp Biol Med (Maywood). 2008 Jul;233(7):897-900. doi: 10.3181/0802-RM-43. Epub 2008 Apr 29.
4
Activation of adenosine low-affinity A3 receptors inhibits the enteric short interplexus neural circuit triggered by histamine.
Am J Physiol Gastrointest Liver Physiol. 2009 Dec;297(6):G1147-62. doi: 10.1152/ajpgi.00295.2009. Epub 2009 Oct 1.
5
Cannabinoid receptor 2 and its agonists mediate hematopoiesis and hematopoietic stem and progenitor cell mobilization.
Blood. 2011 Jan 20;117(3):827-38. doi: 10.1182/blood-2010-01-265082. Epub 2010 Nov 9.
6
Stimulation of the adenosine A3 receptor, not the A1 or A2 receptors, promote neurite outgrowth of retinal ganglion cells.
Exp Eye Res. 2018 May;170:160-168. doi: 10.1016/j.exer.2018.02.019. Epub 2018 Feb 24.
8
IB-MECA, an adenosine A(3) receptor agonist, does not influence survival of lethally γ-irradiated mice.
Physiol Res. 2012;61(6):649-54. doi: 10.33549/physiolres.932411. Epub 2012 Oct 25.

引用本文的文献

1
Extracellular adenosine oppositely regulates the purinome machinery in glioblastoma and mesenchymal stem cells.
IUBMB Life. 2024 Dec;76(12):1234-1251. doi: 10.1002/iub.2905. Epub 2024 Aug 12.
3
Metabolic regulation of skeletal cell fate and function in physiology and disease.
Nat Metab. 2021 Jan;3(1):11-20. doi: 10.1038/s42255-020-00321-3. Epub 2021 Jan 4.
4
The role of purinergic receptors in stem cell differentiation.
Comput Struct Biotechnol J. 2014 Nov 7;13:75-84. doi: 10.1016/j.csbj.2014.11.003. eCollection 2015.
5
Enhanced survival of lethally irradiated adenosine A3 receptor knockout mice. A role for hematopoietic growth factors?
Purinergic Signal. 2015 Mar;11(1):79-85. doi: 10.1007/s11302-014-9432-5. Epub 2014 Oct 31.
6
Lack of adenosine A3 receptors causes defects in mouse peripheral blood parameters.
Purinergic Signal. 2014 Sep;10(3):509-14. doi: 10.1007/s11302-014-9412-9. Epub 2014 Apr 25.

本文引用的文献

1
Expression of mRNA for adenosine A(1), A(2a), A(2b), and A(3) receptors in HL-60 cells: dependence on cell cycle phases.
Physiol Res. 2011;60(6):913-20. doi: 10.33549/physiolres.932233. Epub 2011 Oct 12.
2
Hematopoietic stem cells survive circulation arrest and reconstitute hematopoiesis in myeloablated mice.
Biol Blood Marrow Transplant. 2011 Sep;17(9):1273-81. doi: 10.1016/j.bbmt.2011.07.007. Epub 2011 Jul 20.
3
The role of adenosine receptor agonists in regulation of hematopoiesis.
Molecules. 2011 Jan 17;16(1):675-85. doi: 10.3390/molecules16010675.
5
Homeostatic action of adenosine A3 and A1 receptor agonists on proliferation of hematopoietic precursor cells.
Exp Biol Med (Maywood). 2008 Jul;233(7):897-900. doi: 10.3181/0802-RM-43. Epub 2008 Apr 29.
6
Adenosine A(3) receptor agonist acts as a homeostatic regulator of bone marrow hematopoiesis.
Biomed Pharmacother. 2007 Jul;61(6):356-9. doi: 10.1016/j.biopha.2007.02.010. Epub 2007 Mar 8.
7
Effects of adenosine A(3) receptor agonist on bone marrow granulocytic system in 5-fluorouracil-treated mice.
Eur J Pharmacol. 2006 May 24;538(1-3):163-7. doi: 10.1016/j.ejphar.2006.03.042. Epub 2006 Mar 24.
9
N6-cyclopentyladenosine inhibits proliferation of murine haematopoietic progenitor cells in vivo.
Eur J Pharmacol. 2005 Jan 10;507(1-3):1-6. doi: 10.1016/j.ejphar.2004.11.027. Epub 2004 Dec 15.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验