Laboratory of Experimental Hematology, Department of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Physiol Res. 2011;60(6):913-20. doi: 10.33549/physiolres.932233. Epub 2011 Oct 12.
The present studies investigated changes in expression of mRNA for adenosine A(1), A(2a), A(2b), and A(3) receptors in samples of HL-60 promyelocytic cells differing in the actual presence of cells in various phases of the cell cycle induced by the double thymidine block method. Real-time PCR technique was used for obtaining data on mRNA expression. Statistical analysis of the data revealed that the mRNA expression of adenosine A(1), A(2a), and A(3) receptors is dependent on the cell cycle phase. G(0)/G(1) and G(2)/M phases were characterized by a higher mRNA expression of adenosine A(1) receptors and a lower one of adenosine A(2a) and A(3) receptors whereas the opposite was true for the S phase. Interestingly, expression of mRNA of the adenosine A(2b) receptors was independent on the cell cycle phase. The results indicate the plasticity of mRNA expression of adenosine receptors in the investigated promyelocytic cells and its interaction with physiological mechanisms of the cell cycle.
本研究调查了在双胸苷阻断法诱导的不同细胞周期阶段的 HL-60 早幼粒细胞细胞样本中，腺苷 A(1)、A(2a)、A(2b)和 A(3)受体的 mRNA 表达变化。实时 PCR 技术用于获得 mRNA 表达数据。数据分析显示，腺苷 A(1)、A(2a)和 A(3)受体的 mRNA 表达依赖于细胞周期阶段。G0/G1 和 G2/M 期的腺苷 A(1)受体 mRNA 表达较高，而腺苷 A(2a)和 A(3)受体的 mRNA 表达较低，而 S 期则相反。有趣的是，腺苷 A(2b)受体的 mRNA 表达与细胞周期阶段无关。研究结果表明，所研究的早幼粒细胞中腺苷受体的 mRNA 表达具有可塑性，并与其细胞周期的生理机制相互作用。
