oriC DNA与DnaA起始蛋白的复合物。

The complex of oriC DNA with the DnaA initiator protein.

作者信息

Messer W, Egan B, Gille H, Holz A, Schaefer C, Woelker B

机构信息

Max-Planck-Institut für molekulare Genetik, Berlin, Germany.

出版信息

Res Microbiol. 1991 Feb-Apr;142(2-3):119-25. doi: 10.1016/0923-2508(91)90018-6.

DOI:10.1016/0923-2508(91)90018-6

PMID:1925008

Abstract

We describe several experimental approaches relating to the early steps in the initiation of DNA replication at oriC. 1) A matrix is given which enables calculatation of the relative affinity of DnaA boxes for DnaA protein; 2) base changes within single Dna A boxes in oriC have little effect on oriC function; 3) mutations which change the distance between DnaA boxes inactivate oriC, but changes by one helical turn (+ and -) result in near wild-type oriC activity; 4) a Fis binding site was located at oriC coordinates 206-220; 5) KMnO4 probing demonstrates Dna-A-dependent unwinding in the left part of oriC in vivo and in vitro.

摘要

我们描述了几种与oriC处DNA复制起始早期步骤相关的实验方法。1）给出了一个矩阵，可用于计算DnaA框对DnaA蛋白的相对亲和力；2）oriC中单个DnaA框内的碱基变化对oriC功能影响很小；3）改变DnaA框之间距离的突变会使oriC失活，但增加或减少一个螺旋圈的变化会导致接近野生型的oriC活性；4）在oriC坐标206 - 220处定位到一个Fis结合位点；5）高锰酸钾探测表明，在体内和体外，oriC左侧部分存在依赖Dna - A的解旋。