缺乏类组蛋白H-NS的大肠杆菌δhns突变体产生无核细胞
Anucleate cell production by Escherichia coli delta hns mutant lacking a histone-like protein, H-NS.
作者信息
Kaidow A, Wachi M, Nakamura J, Magae J, Nagai K
机构信息
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
J Bacteriol. 1995 Jun;177(12):3589-92. doi: 10.1128/jb.177.12.3589-3592.1995.
Abstract
Normal-sized anucleate cells were observed in the cultures of a delta hns mutant strain. Even in nucleate cells, some populations showed irregular intracellular localization of the nucleoids. The delta hns mutant showed reduced ploidy, although initiation of chromosome replication was essentially synchronous as defined by flow cytometric analysis. These results indicate that the delta hns mutant is defective in the mechanisms of chromosome partitioning and chromosome replication.
摘要
在δhns突变菌株的培养物中观察到正常大小的无核细胞。即使在有核细胞中，一些群体的类核在细胞内的定位也不规则。尽管通过流式细胞术分析确定染色体复制的起始基本同步，但δhns突变体的倍性降低。这些结果表明，δhns突变体在染色体分配和染色体复制机制方面存在缺陷。
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