Stein Gary S, Zaidi Sayyed K, Stein Janet L, Lian Jane B, van Wijnen Andre J, Montecino Martin, Young Daniel W, Javed Amjad, Pratap Jitesh, Choi Je-Yong, Ali Syed A, Pande Sandhya, Hassan Mohammad Q
University of Massachusetts Medical School, Department of Cell Biology and Cancer Center, Worcester, Massachusetts 01655, USA.
Ann N Y Acad Sci. 2009 Feb;1155:4-14. doi: 10.1111/j.1749-6632.2009.03697.x.
There is growing awareness that the fidelity of gene expression necessitates coordination of transcription factor metabolism and organization of genes and regulatory proteins within the three-dimensional context of nuclear architecture. The regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex organization and assembly are functionally linked to biological control and are compromised with the onset and progression of tumorigenesis. High throughput imaging of cells, tissues, and tumors, including live cell analysis, is expanding research's capabilities toward translating components of nuclear organization into novel strategies for cancer diagnosis and therapy.
人们越来越意识到，基因表达的保真度需要在核结构的三维背景下协调转录因子代谢以及基因和调节蛋白的组织。控制基因表达的遗传和表观遗传调控机制在核微环境中是分隔的。调节复合物组织和组装的时间和空间参数在功能上与生物控制相关联，并随着肿瘤发生的开始和进展而受到损害。对细胞、组织和肿瘤的高通量成像，包括活细胞分析，正在扩展研究能力，以将核组织成分转化为癌症诊断和治疗的新策略。
