Aihara Hiroyuki, Sumiyama Kazuki, Saito Shoichi, Tajiri Hisao, Ikegami Masahiro
Department of Endoscopy, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.
Gastrointest Endosc. 2009 Mar;69(3 Pt 2):726-33. doi: 10.1016/j.gie.2008.10.044.
Autofluorescence endoscopy (AFE) may improve detection and diagnosis of colorectal lesions. Recently, AFE based on a high-resolution video endoscope was developed.
A novel high-resolution video AFE system was used to quantify autofluorescence of colorectal lesions to determine the characteristics of non-neoplastic and neoplastic lesions.
Retrospective observational study.
Single-center referral hospital.
Ninety-seven patients with 103 colorectal lesions (22 non-neoplastic and 81 neoplastic lesions) who underwent AFE and were treated by using endoscopy or by surgery.
Recorded digital AFE images were analyzed to quantify autofluorescence. The following autofluorescence indexes were calculated: the green/red (G/R) ratio for each lesion, the color-contrast index between each lesion, and the corresponding normal region.
The G:R ratio, color-contrast index, and histopathologic characteristics for each colorectal lesion.
The mean G/R ratio was significantly higher in non-neoplastic lesions (1.17 [95% CI, 1.10-1.24], n = 22) than in neoplastic lesions (0.65 [95% CI, 0.63-0.68], n = 81) (P < .001). Mean color-contrast indexes were significantly lower in non-neoplastic lesions (7.99 [95% CI, 6.40-9.58], n = 22) than neoplastic lesions (35.06 [95% CI, 32.79-37.33], n = 81; P < .001). With a cutoff value of 1.01 for the G/R ratio and 13.94 for color-contrast index, AFE had a sensitivity and specificity of 98.8% and 86.4% respectively, for G/R ratio, and 98.8% and 90.9%, respectively, for color contrast index, in differentiating neoplastic from non-neoplastic colorectal lesions.
Retrospective design.
The quantification of digital AFE images obtained from the novel high-resolution videoendoscopy system revealed that autofluorescence was significantly different between non-neoplastic and neoplastic lesions, and color tone in AFE may represent the histopathologic characteristics of the lesion.
自体荧光内镜检查（AFE）可能会改善大肠病变的检测与诊断。最近，基于高分辨率视频内镜的AFE被开发出来。
使用一种新型高分辨率视频AFE系统对大肠病变的自体荧光进行量化，以确定非肿瘤性和肿瘤性病变的特征。
回顾性观察研究。
单中心转诊医院。
97例患有103处大肠病变（22处非肿瘤性病变和81处肿瘤性病变）的患者，这些患者接受了AFE检查，并通过内镜检查或手术进行治疗。
对记录的数字AFE图像进行分析以量化自体荧光。计算以下自体荧光指数：每个病变的绿/红（G/R）比值、每个病变与其相应正常区域之间的颜色对比指数。
每个大肠病变的G:R比值、颜色对比指数和组织病理学特征。
非肿瘤性病变（1.17[95%CI，1.10 - 1.24]，n = 22）的平均G/R比值显著高于肿瘤性病变（0.65[95%CI，0.63 - 0.68]，n = 81）（P <.001）。非肿瘤性病变的平均颜色对比指数（7.99[95%CI，6.40 - 9.58]，n = 22）显著低于肿瘤性病变（35.06[95%CI，32.79 - 37.33]，n = 81；P <.001）。以G/R比值的临界值1.01和颜色对比指数的临界值13.94为标准，在区分肿瘤性与非肿瘤性大肠病变时，AFE对于G/R比值的敏感性和特异性分别为98.8%和86.4%，对于颜色对比指数的敏感性和特异性分别为98.8%和90.9%。
回顾性设计。
从新型高分辨率视频内镜系统获得的数据AFE图像量化显示，非肿瘤性和肿瘤性病变之间的自体荧光存在显著差异，AFE中的色调可能代表病变的组织病理学特征。
