Ohsaki Yoshinobu, Sasaki Takaaki, Endo Satoshi, Kitada Masahiro, Okumura Shunsuke, Hirai Noriko, Kazebayashi Yoshihiro, Toyoshima Eri, Yamamoto Yasushi, Takeyama Kaneyoshi, Nakajima Susumu, Sakata Isao
Respiratory Center, Asahikawa Medical University, 2-1-1-1 Midorigaoka Higashi, Asahikawa, 078-8510, Japan.
Moriyama Memorial Hospital, Asahimachi 2-1-31, Asahikawa, 070-0832, Japan.
BMC Cancer. 2017 Apr 26;17(1):289. doi: 10.1186/s12885-017-3277-6.
We observed red autofluorescence emanating from bronchial cancer lesions using a sensitive color-fluorescence endoscopy system. We investigated to clarify the origin of the red autofluorescence.
The wavelengths of the red autofluorescence emanating from lesions were measured in eight patients using a spectrum analyzer and compared based on pathologic findings. Red autofluorescence at 617.3, 617.4, 619.0, and 617.1 nm was emitted by normal bronchus, inflamed tissue, tissue exhibiting mild dysplasia, and malignant lesions, respectively. Protoporphyrin, uroporphyrin, and coproporphyrin, the major porphyrin derivatives in human blood, were purchased to determine which porphyrin derivative is the source of red fluorescence when acquired de novo. We synthesized photoporphyrin, Zn-protoporphyrin and Zn-photoprotoporphyrin from protoporphyrin.
Coproporphyrin and uroporphyrin emitted only weak fluorescence. Fluorescence was emitted by our synthesized Zn-photoprotoporphyrin at 625.5 nm and by photoprotoporphyrin at 664.0 nm.
From these results, we conclude that Zn-photoprotoporphyrin was the source of the red autofluorescence observed in bronchial lesions. Zn-protoporphyrin is converted to Zn-photoprotoporphyrin by radiation with excitation light. Our results suggest that red autofluorescence emanating from Zn-photoprotoporphyrin in human tissues could interfere with photodynamic diagnosis using porphyrin derivatives such as Photofrin® and Lazerphyrin® with a sensitive endoscopy system, because color cameras cannot differentiate Zn-photoprotoporphyrin red fluorescence from that of other porphyrin derivatives.
我们使用一种灵敏的彩色荧光内镜系统观察到支气管癌病变发出红色自发荧光。我们进行研究以阐明红色自发荧光的来源。
使用光谱分析仪测量了8例患者病变发出的红色自发荧光的波长，并根据病理结果进行比较。正常支气管、炎症组织、轻度发育异常组织和恶性病变分别发出波长为617.3、617.4、619.0和617.1nm的红色自发荧光。购买了人体血液中的主要卟啉衍生物原卟啉、尿卟啉和粪卟啉，以确定重新获得时哪种卟啉衍生物是红色荧光的来源。我们从原卟啉合成了光卟啉、锌原卟啉和锌光原卟啉。
粪卟啉和尿卟啉仅发出微弱荧光。我们合成的锌光原卟啉在625.5nm处发出荧光，光卟啉在664.0nm处发出荧光。
根据这些结果，我们得出结论，锌光原卟啉是在支气管病变中观察到的红色自发荧光的来源。锌原卟啉通过激发光照射转化为锌光原卟啉。我们的结果表明，人体组织中锌光原卟啉发出的红色自发荧光可能会干扰使用诸如Photofrin®和Lazerphyrin®等卟啉衍生物通过灵敏内镜系统进行的光动力诊断，因为彩色相机无法区分锌光原卟啉的红色荧光与其他卟啉衍生物的红色荧光。
