Muhonen Jaana, Holopainen Juha M, Wiedmer Susanne K
Department of Chemistry, Laboratory of Analytical Chemistry, University of Helsinki, Helsinki, Finland.
J Chromatogr A. 2009 Apr 10;1216(15):3392-7. doi: 10.1016/j.chroma.2009.02.028. Epub 2009 Feb 21.
In the case of local anesthetic intoxication, intravenous administration of lipid-based Intralipid dispersion (Fresenius Kabi) can be used for the entrapment of hydrophobic drugs. Our long-term aim is to develop a sensitive, efficient, and non-harmful lipid-based formulation to specifically trap harmful substances. In this study liposome electrokinetic capillary chromatography (LEKC) was used to study the interactions between local anesthetics and Intralipid or liposome dispersions. Intralipid dispersion and extruded liposomes with different concentrations of 1-palmitoyl-2-oleyl-sn-glycerophosphatidylcholine (POPC), phosphatidylglycerol, cardiolipin, cholesterol, oleic acid, and linoleic acid were used as a pseudostationary phase in LEKC and their interactions with lidocaine, prilocaine, and bupivacaine were studied. POPC liposomes containing 1mol% of palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphocholine as a fluorescent marker were used for the first time in LEKC connected with laser-induced fluorescent detection in order to calculate the retention factor for anesthetics.
在局部麻醉药中毒的情况下，静脉注射基于脂质的英脱利匹特乳剂（费森尤斯卡比公司生产）可用于捕获疏水性药物。我们的长期目标是开发一种灵敏、高效且无害的基于脂质的制剂，以特异性地捕获有害物质。在本研究中，脂质体电动毛细管色谱法（LEKC）被用于研究局部麻醉药与英脱利匹特或脂质体乳剂之间的相互作用。不同浓度的1-棕榈酰-2-油酰-sn-甘油磷脂酰胆碱（POPC）、磷脂酰甘油、心磷脂、胆固醇、油酸和亚油酸的英脱利匹特乳剂和挤压脂质体在LEKC中用作假固定相，并研究了它们与利多卡因、丙胺卡因和布比卡因的相互作用。首次在与激光诱导荧光检测联用的LEKC中使用含有1mol%棕榈酰-2-[12-[(7-硝基-2-1,3-苯并恶二唑-4-基)氨基]十二烷酰]-sn-甘油-3-磷酸胆碱作为荧光标记物的POPC脂质体，以计算麻醉药的保留因子。
