Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, 00014 Helsinki, Finland.
Anal Bioanal Chem. 2010 Apr;396(7):2599-607. doi: 10.1007/s00216-009-3435-z. Epub 2010 Feb 7.
Interactions between Intralipid dispersion and local anesthetics (bupivacaine, prilocaine, and lidocaine) were investigated. The amount of bupivacaine (the most cardiotoxic analyte of the local anesthetics studied) entrapped in Intralipid in the presence of plasma was studied using an off-line filtration and solid phase extraction method combined with capillary zone electrophoresis for quantification of free unbound bupivacaine. To confirm interactions between the analytes and Intralipid at lower concentrations, direct injection mass spectrometry was used. The use of immobilized Intralipid chromatography-atmospheric pressure ionization-ion trap mass spectrometry in the study of interactions between drugs and Intralipid dispersion is demonstrated. Finally, interactions between Intralipid dispersion and local anesthetics were investigated by electrokinetic capillary chromatography. The electrophoretic mobility of the Intralipid dispersed phase was calculated using the iterative procedure and a homologous series of alkyl phenyl benzoates (C(1)-C(6)), and the retention factors for the analytes were determined.
研究了脂肪乳剂分散相与局部麻醉剂（布比卡因、普鲁卡因和利多卡因）之间的相互作用。采用离线过滤和固相萃取方法结合毛细管区带电泳，研究了在血浆存在下布比卡因（研究的局部麻醉剂中最具心脏毒性的分析物）在脂肪乳剂中的包封量。为了证实分析物与脂肪乳剂在较低浓度下的相互作用，直接注射质谱法被用于分析。采用固定化脂肪乳剂色谱-大气压电离-离子阱质谱法研究了药物与脂肪乳剂分散体之间的相互作用。最后，通过电泳毛细管色谱法研究了脂肪乳剂分散相与局部麻醉剂之间的相互作用。采用迭代程序和一系列烷基苯基苯甲酸酯（C（1）-C（6））计算了脂肪乳剂分散相的电泳迁移率，并确定了分析物的保留因子。
