罗红霉素抑制趋化因子诱导的Th1和Th2细胞的趋化作用，但不影响调节性T细胞。

Roxithromycin inhibits chemokine-induced chemotaxis of Th1 and Th2 cells but regulatory T cells.

作者信息

Ito Taisuke, Ito Natsuho, Hashizume Hideo, Takigawa Masahiro

机构信息

Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

出版信息

J Dermatol Sci. 2009 Jun;54(3):185-91. doi: 10.1016/j.jdermsci.2009.01.007. Epub 2009 Feb 28.

DOI:10.1016/j.jdermsci.2009.01.007

PMID:19251402

Abstract

BACKGROUND

Roxithromycin (RXM), a 14-member macrolide antibiotic, has a variety of bioregulatory functions such as anti-inflammatory effects, anti-oxidant effects, and modulation of immune responses.

OBJECTIVES

In this study, we analyzed the effect of RXM on chemokine-induced chemotaxis of Th1, Th2, and regulatory T (Treg) cells established from three normal human peripheral blood lymphocytes by the reported methods.

METHODS AND RESULTS

Incubation with 10 microM RXM for 18 h did not alter the expression profile of CXCR3 on Th1 cells and CCR4 on Th2 and Treg cells. However, upon RXM preincubation, the migration of Th1 cells to IP-10 and Th2 cells to TARC was partially suppressed, although RXM did not influence Treg cell migration. Erythromycin and clarithromycin at the same concentration did not exert such effects. F-actin polymerization and Ca(++) influx induced by IP-10 and TARC in Th1 and Th2 cells, respectively, was down-regulated by RXM pretreatment.

CONCLUSION

These results imply that RXM exhibits bioregulatory function by influencing chemotaxis of Th1 and Th2 cells while leaving Treg cell migration unaffected.

摘要

背景

罗红霉素（RXM）是一种14元大环内酯类抗生素，具有多种生物调节功能，如抗炎作用、抗氧化作用和免疫反应调节作用。

目的

在本研究中，我们通过报道的方法分析了RXM对从三名正常人类外周血淋巴细胞建立的Th1、Th2和调节性T（Treg）细胞趋化因子诱导的趋化作用的影响。

方法与结果

用10μM RXM孵育18小时不会改变Th1细胞上CXCR3以及Th2和Treg细胞上CCR4的表达谱。然而，在RXM预孵育后，Th1细胞向IP-10的迁移以及Th2细胞向TARC的迁移受到部分抑制，尽管RXM不影响Treg细胞迁移。相同浓度的红霉素和克拉霉素没有发挥这种作用。RXM预处理分别下调了IP-10和TARC在Th1和Th2细胞中诱导的F-肌动蛋白聚合和Ca(++)内流。