基于TaqMan探针的实时荧光定量PCR检测方法，用于检测和区分经苯氧羧酸类除草剂处理的土壤中的I类、II类和III类tfdA基因。
TaqMan probe-based real-time PCR assay for detection and discrimination of class I, II, and III tfdA genes in soils treated with phenoxy acid herbicides.
作者信息
Baelum Jacob, Jacobsen Carsten S
机构信息
Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark.
出版信息
Appl Environ Microbiol. 2009 May;75(9):2969-72. doi: 10.1128/AEM.02051-08. Epub 2009 Feb 27.
Abstract
Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.
摘要
在三种苯氧基酸矿化之后，使用TaqMan定量实时PCR对13种不同土壤中三类tfdA基因进行了单独定量分析。结果发现，III类tfdA基因比I类和II类tfdA基因更常参与矿化过程。
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