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冷冻对与珊瑚黑带病相关微生物的16S rRNA基因PCR扩增的影响。

Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals.

作者信息

Sekar Raju, Kaczmarsky Longin T, Richardson Laurie L

机构信息

Department of Biological Sciences, Florida International University, Miami, Florida 33199, USA.

出版信息

Appl Environ Microbiol. 2009 Apr;75(8):2581-4. doi: 10.1128/AEM.01500-08. Epub 2009 Feb 27.

DOI:10.1128/AEM.01500-08
PMID:19251896
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2675240/
Abstract

Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

摘要

珊瑚黑带病的分子分析表明，采集后立即冷冻的样本产生了更多的变形菌门16S rRNA序列，而未冷冻的样本产生了更多的蓝细菌和硫氧化细菌序列。这些结果表明，需要采用多种样本制备方法来表征这种复杂的多微生物疾病。

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