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基于 PCR 的真核生物相关细菌群落结构研究：一种避免真核 DNA 共扩增的简单 PCR 策略。

PCR-based community structure studies of bacteria associated with eukaryotic organisms: a simple PCR strategy to avoid co-amplification of eukaryotic DNA.

机构信息

Norwegian University of Science and Technology, Department of Biotechnology, Trondheim, Norway.

出版信息

J Microbiol Methods. 2011 Feb;84(2):349-51. doi: 10.1016/j.mimet.2010.12.015. Epub 2010 Dec 21.

DOI:10.1016/j.mimet.2010.12.015
PMID:21182876
Abstract

PCR primers targeting conserved regions of the SSU rRNA gene are commonly used in bacterial community studies. For microbes associated with eukaryotes, co-amplification of eukaryotic DNA may preclude the analysis. We present a simple and efficient PCR strategy to obtain pure bacterial rDNA amplicons from samples predominated by eukaryotic DNA.

摘要

针对 16S rRNA 基因保守区的 PCR 引物通常用于细菌群落研究。对于与真核生物相关的微生物，真核生物 DNA 的共扩增可能会妨碍分析。我们提出了一种简单有效的 PCR 策略，从以真核生物 DNA 为主的样品中获得纯细菌 rDNA 扩增子。

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