Funahashi Tatsuya, Tanabe Tomotaka, Shiuchi Keiichi, Nakao Hiroshi, Yamamoto Shigeo
College of Pharmaceutical Sciences, Matsuyama University, Japan.
Biol Pharm Bull. 2009 Mar;32(3):359-65. doi: 10.1248/bpb.32.359.
During the course of our investigation on the iron acquisition systems in Vibrio parahaemolyticus, a causative agent of seafood-related gastroenteritis, we found that this species utilizes desferri-ferrichrome for growth as a heterologous siderophore (a siderophore produced by other bacteria and fungi) under iron-limiting conditions. N-Terminal amino acid sequence analysis of the iron-repressible outer membrane proteins followed by searches of the reported genomic sequences of this species identified four relevant genes (called fhuACDB) forming an operon. Deletion analysis of the fhuA and fhuD genes indicated that the most upstream gene fhuA and the three downstream genes fhuCDB encode the ferrichrome receptor and the ATP-binding cassette transport components, respectively. Moreover, it was found that the fhuCDB genes are also required for transport of ferric aerobactin which restores growth of this species under iron-limiting conditions.
在我们对与海鲜相关的肠胃炎病原体副溶血性弧菌的铁获取系统进行研究的过程中，我们发现该物种在铁限制条件下利用去铁铁载体作为异源铁载体（由其他细菌和真菌产生的铁载体）进行生长。对铁抑制性外膜蛋白进行N端氨基酸序列分析，随后搜索该物种已报道的基因组序列，鉴定出四个相关基因（称为fhuACDB）形成一个操纵子。对fhuA和fhuD基因的缺失分析表明，最上游的基因fhuA和三个下游基因fhuCDB分别编码铁载体受体和ATP结合盒转运组件。此外，还发现fhuCDB基因对于高铁嗜铁素的转运也是必需的，高铁嗜铁素可在铁限制条件下恢复该物种的生长。
