McIntosh Kathleen A, Harding John C S, Parker Sarah, Krakowka Steven, Allan Gordon, Ellis John A
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada.
Can Vet J. 2008 Dec;49(12):1189-94.
This study examined if pigs in a Porcine circovirus disease (PCVD)-affected herd (n = 100) had shed more Porcine circovirus-2 (PCV-2) in their feces than pigs in a PCVD-nonaffected herd (n = 101), and if differences in shedding among production stages within and between the herds existed. The PCV-2 shedding was quantified by real-time polymerase chain reaction. The highest median PCV-2 shedding was found in the nursery of the PCVD-affected herd and in the grower of the PCVD-nonaffected herd. The PCV-2 shedding was significantly higher in earlier stages (newly weaned, nursery, and pregrower) in the PCVD-affected herd (Wilcoxon rank sum; P < 0.001) compared with the PCVD-nonaffected herd. Porcine circovirus-2 DNA was not detected in a significant proportion of lactating sows (parity > or = 3) in the PCVD-nonaffected herd (Fisher's exact test; P = 0.001). The results of this study suggest there may be an association between the presence of PCV-2 in the feces of lactating sows and increased PCV-2 shedding in younger pigs.
本研究调查了猪圆环病毒病（PCVD）感染猪群（n = 100）中的猪粪便中猪圆环病毒2型（PCV - 2）的排出量是否高于未感染PCVD的猪群（n = 101）中的猪，以及两个猪群内部和之间不同生产阶段的病毒排出量是否存在差异。通过实时聚合酶链反应对PCV - 2排出量进行定量。在感染PCVD的猪群的保育阶段以及未感染PCVD的猪群的生长育肥阶段发现了最高的PCV - 2排出量中位数。与未感染PCVD的猪群相比，感染PCVD的猪群在早期阶段（刚断奶、保育和生长育肥前期）的PCV - 2排出量显著更高（Wilcoxon秩和检验；P < 0.001）。在未感染PCVD的猪群中，相当比例的经产母猪（胎次≥3）未检测到PCV - 2 DNA（Fisher精确检验；P = 0.001）。本研究结果表明，泌乳母猪粪便中PCV - 2的存在与幼猪PCV - 2排出量增加之间可能存在关联。
