从对甜菜夜蛾核多角体病毒感染高度敏感的甜菜夜蛾细胞系（IOZCAS-Spex-II）克隆得到的一个细胞株。
A cell strain cloned from Spodoptera exigua cell line (IOZCAS-Spex-II) highly susceptible to S. exigua nucleopolyhedrovirus infection.
作者信息
Zhang Huan, Zhang Yong-an, Qin Qilian, Li Xuan, Miao Lin, Wang Yuzhu, Qu Liangjian, Zhang Aijun, Yang Qing
机构信息
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Datunlu, Beijing, 100101, China.
出版信息
In Vitro Cell Dev Biol Anim. 2009 May-Jun;45(5-6):201-4. doi: 10.1007/s11626-009-9177-6. Epub 2009 Feb 28.
A cell strain (IOZCAS-Spex-II-A) cloned from IOZCAS-Spex-II, a cell line established from the fat body of Spodoptera exigua (Lepidoptera: Noctuidae) larva, was characterized, and its capability to produce S. exigua nucleopolyhedrovirus was high with infection rate exceeding 90% compared with its parental cell line IOZCAS-Spex-II that scored only 50%. Growth curve of budded virus (BV) in the strain was analyzed and the titer of BV reached the highest of 3.7 x 10(4) pfu/mL by 96 h after inoculation. Concentration of occlusion bodies (OBs) produced by the cloned cell strain (IOZCAS-Spex-II-A) was 7.1 x 10(7) OBs/mL, while the parental cell line produced 2.4 x 10(7) OBs/mL. The average yield of the virus was 176 OBs/cell of IOZCAS-Spex-II-A compared with 211 OBs/cell that of the parental cell line. Significant differences were observed in virus production, growth characters, cell shape, between the parental cell line, and its clone. The cell lines (IOZCAS-Spex-II and IOZCAS-Spex-II-A) were also susceptible to Autographa californica multiple nucleopolyhedrovirus infection. In addition, they were characterized with regard to their growth rates and DNA amplification fingerprinting technique employing polymerase chain reaction.
从甜菜夜蛾（鳞翅目：夜蛾科）幼虫脂肪体建立的细胞系IOZCAS - Spex - II中克隆得到一个细胞株（IOZCAS - Spex - II - A），对其进行了特性分析，与亲代细胞系IOZCAS - Spex - II相比，其产生甜菜夜蛾核多角体病毒的能力很强，感染率超过90%，而亲代细胞系的感染率仅为50%。分析了该细胞株中出芽病毒（BV）的生长曲线，接种后96小时BV滴度最高达到3.7×10⁴ pfu/mL。克隆细胞株（IOZCAS - Spex - II - A）产生的包涵体（OBs）浓度为7.1×10⁷ OBs/mL，而亲代细胞系产生的为2.4×10⁷ OBs/mL。IOZCAS - Spex - II - A的病毒平均产量为176 OBs/细胞，而亲代细胞系为211 OBs/细胞。在病毒产生、生长特性、细胞形态方面，亲代细胞系与其克隆之间存在显著差异。这两个细胞系（IOZCAS - Spex - II和IOZCAS - Spex - II - A）对苜蓿银纹夜蛾多核多角体病毒感染也敏感。此外，还采用聚合酶链反应对它们的生长速率和DNA扩增指纹技术进行了特性分析。
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