Smith G E, Ju G, Ericson B L, Moschera J, Lahm H W, Chizzonite R, Summers M D
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8404-8. doi: 10.1073/pnas.82.24.8404.
A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.
编码人白细胞介素2（IL-2）的互补DNA（cDNA）被插入到苜蓿银纹夜蛾核型多角体病毒基因组中多角体蛋白启动子附近。用重组病毒感染的细胞产生了高水平的分子量为15500的IL-2多肽，其中大部分在感染过程中分泌到培养基中。重组IL-2能够刺激依赖IL-2的细胞系生长。昆虫来源的IL-2的N端氨基酸序列与天然IL-2相同。因此，哺乳动物的信号肽在昆虫细胞中被识别并正确切除。
