Smith G E, Ju G, Ericson B L, Moschera J, Lahm H W, Chizzonite R, Summers M D
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8404-8. doi: 10.1073/pnas.82.24.8404.
A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.
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