Wang Nan-Nan, Shao Yan-Qing, Tang Yu-Hai, Yin He-Ping, Wu Xiao-Zhong
Department of Medicament, The Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, People's Republic of China.
Luminescence. 2009 May-Jun;24(3):178-82. doi: 10.1002/bio.1092.
A sensitive and simple flow-injection chemiluminescence (FI-CL) method, which was based on the CL intensity generated from the redoxreaction of potassium permanganate (KMnO4)-formaldehyde in vitriol (H2SO4) medium, has been developed, validated and applied for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride. Besides oxidants and sensitizers, the effect of the concentration of H(2)SO(4), KMnO4 and formaldehyde was investigated. Under the optimum conditions, the linear range was 1.0 x 10(-2)-7.0 mg/L for naphazoline hydrochloride and 5.0 x 10(-2)-10.0 mg/L for oxymetazoline hydrochloride. During seven repeated inter-day and intra-day precision tests of 0.1, 1.0 and 10.0 mg/L samples, the relative standard deviations all corresponded to reference values. The detection limit was 8.69 x 10(-3) mg/L for naphazoline hydrochloride and 3.47 x 10(-2) mg/L for oxymetazoline hydrochloride (signal-to-noise ratio < or = 3). This method has been successfully implemented for the determination of naphazoline hydrochloride and oxymetazoline hydrochloride in pharmaceuticals.
基于高锰酸钾（KMnO₄）-甲醛在硫酸（H₂SO₄）介质中氧化还原反应产生的化学发光强度，开发、验证并应用了一种灵敏且简便的流动注射化学发光（FI-CL）方法，用于测定盐酸萘甲唑啉和盐酸羟甲唑啉。除了氧化剂和增敏剂外，还研究了硫酸、高锰酸钾和甲醛浓度的影响。在最佳条件下，盐酸萘甲唑啉的线性范围为1.0×10⁻² - 7.0 mg/L，盐酸羟甲唑啉的线性范围为5.0×10⁻² - 10.0 mg/L。在对0.1、1.0和10.0 mg/L样品进行的七次重复日间和日内精密度测试中，相对标准偏差均符合参考值。盐酸萘甲唑啉的检测限为8.69×10⁻³ mg/L，盐酸羟甲唑啉的检测限为3.47×10⁻² mg/L（信噪比≤3）。该方法已成功用于测定药品中的盐酸萘甲唑啉和盐酸羟甲唑啉。
