Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran.
Luminescence. 2014 Feb;29(1):48-51. doi: 10.1002/bio.2500. Epub 2013 Mar 6.
A simple and sensitive flow injection chemiluminescence (FI-CL) method was developed for the determination of naphazoline hydrochloride (NPZ). The method is based on the enhancing effect of NPZ on the weak CL signal from the reaction of KIO4 with H2 O2 . Experimental parameters that affected the CL signal, including the pH of the KIO4 solution, concentrations of KIO4 , H2 O2 and disodium-EDTA and flow rate were optimized. Under the optimum conditions, the increment of CL intensity was linearly proportional to the concentration of NPZ in the range 5.0 × 10(-6) to 70 × 10(-6) mol/L. The detection limit was 1.0 × 10(-6) mol/L and the relative standard deviation for 50 × 10(-6) mol/L NPZ solution was 2.8% (n = 11). In addition, a high throughput of 120 samples/h was achieved. The utility of this method was demonstrated by determining NPZ in pharmaceuticals.
建立了一种简单、灵敏的流动注射化学发光（FI-CL）法测定盐酸萘甲唑啉（NPZ）。该方法基于 NPZ 增强 KIO4 与 H2O2 反应产生的弱 CL 信号。实验优化了影响 CL 信号的参数，包括 KIO4 溶液的 pH 值、KIO4、H2O2 和乙二胺四乙酸二钠的浓度以及流速。在最佳条件下，CL 强度的增量与 NPZ 的浓度在 5.0×10（-6）至 70×10（-6）mol/L 范围内呈线性关系。检测限为 1.0×10（-6）mol/L，50×10（-6）mol/L NPZ 溶液的相对标准偏差为 2.8%（n=11）。此外，实现了 120 个样品/小时的高通量。该方法已用于测定药物中的 NPZ。
