Chen Yi-Hua, Lu Yu-Fen, Ko Ting-Yi, Tsai Ming-Yuan, Lin Che Yi, Lin Chia-Chi, Hwang Sheng-Ping L
Institute of Cellular and Organismic Biology (formerly Institute of Zoology), Academia Sinica, Nankang, Taipei, Taiwan, ROC.
Dev Dyn. 2009 May;238(5):1021-32. doi: 10.1002/dvdy.21908.
Both antisense morpholino oligonucleotide (MO)-mediated knockdown and overexpression experiments were performed to analyze zebrafish cdx1b's function in intestinal cell differentiation. Substantial reductions in goblet cell numbers were detected in intestines of 102- and 120-hours post-fertilization (hpf) cdx1b MO-injected embryos (morphants) compared to cdx1b-4-base mismatched (4mm)-MO-injected and wild type embryos. A significant decrease in enteroendocrine cell numbers was also observed in intestines of 96-hpf cdx1b morphants. Furthermore, ectopic cdx1b expression caused notable increases in respective cell numbers of enteroendocrine and goblet cells in intestines of 96- and 98-hpf injected embryos. Decreased PepT1 expression was detected in enterocytes of intestines in cdx1b morphants from 80 to 102 hr of development. In addition, increased cell proliferation was detected in intestines of cdx1b morphants. Overall, our results suggest that zebrafish cdx1b plays important roles in regulating intestinal cell proliferation and the differentiation of various intestinal cell lineages.
为了分析斑马鱼cdx1b在肠道细胞分化中的功能，我们进行了反义吗啉代寡核苷酸（MO）介导的敲低和过表达实验。与注射cdx1b-4碱基错配（4mm）-MO的胚胎和野生型胚胎相比，在受精后102小时和120小时（hpf）注射cdx1b MO的胚胎（形态突变体）的肠道中，杯状细胞数量大幅减少。在96 hpf的cdx1b形态突变体的肠道中，也观察到肠内分泌细胞数量显著减少。此外，在96和98 hpf注射胚胎的肠道中，异位表达cdx1b导致肠内分泌细胞和杯状细胞的各自细胞数量显著增加。在发育80至102小时的cdx1b形态突变体的肠道肠细胞中，检测到PepT1表达降低。此外，在cdx1b形态突变体的肠道中检测到细胞增殖增加。总体而言，我们的结果表明，斑马鱼cdx1b在调节肠道细胞增殖和各种肠道细胞谱系的分化中起重要作用。
