[Platinum tetrachloride coupled PEG-PEI as drug carrier and gene delivery vector].
作者信息
Zhao Lu, Chen Huan, Chen Dan, Hu Yi-Ping, Yu Hai, Wang Qing-Qing, Tang Gu-Ping, Hu Xiu-Rong
机构信息
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):59-66. doi: 10.3785/j.issn.1008-9292.2009.01.009.
OBJECTIVE
To construct a drug carrier and gene vector PEG-PEI-Pt.
METHODS
Polyethyleneglycol (PEG) was coupled to polyethylenimine (PEI 600) and platinum tetrachloride; PEG-PEI-Pt complex was formed in ethanol. The complex was characterized by XRD, UV-VIS and FT-IR and the DNA condensation was tested by electrophoretic mobility shift assay. The cell viability was evaluated by MTT assay in Hela, B16, A293 and COS-7 cells and in vitro transfection efficiency was measured in A293 and B16 cells.
RESULT
The structure of PEG-PEI-Pt was characterized by XRD, UV-VIS and FT-IR. PEG-PEI-Pt complex was able to bind DNA at N/P weight ratio of 0.4:1; the complex showed cytotoxicity on Hela and B16 cells. The complex had higher transfection efficiency in A293 and B16 cells than PEI 600.
CONCLUSION
A novel drug carrier and gene vector PEG-PEI-Pt was constructed successfully.
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