香菇多糖-嫁接-聚乙烯亚胺——一种新型基因递送载体
[Lentinan-graft-polyethylenimine-a novel vector for gene delivery].
作者信息
Jiang Qi-Ying, Lu Xiao, Hu Yi-Ping, Chen Dan, Zhao Dan-Jun, Zhou Jun, Yu Hai, Wang Qing-Qing, Tang Gu-Ping
机构信息
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):39-45. doi: 10.3785/j.issn.1008-9292.2009.01.006.
OBJECTIVE
To develop a novel vector for gene delivery with low molecular weight polyethylenimine grafted to the natural polysaccharide and conjugated to folic acid (LNT-PEI-FA).
METHODS
The properties of LNT-PEI-FA were characterized by (1)H-NMR, FT-IR and TGA, respectively. The particle size of LNT-PEI-FA/DNA complex was measured. The DNA binding ability of LNT-PEI-FA was detected by gel electrophoresis retardation assay.
RESULT
The particle size of LNT-PEI-FA/DNA complex was about 200 nm. Gel electrophoresis showed that at N/P ratio of 1.8 (W/W) the polymer was able to completely condense DNA. In vitro experiments showed a high efficiency of gene transfection in A293 and B16 cell lines.
CONCLUSION
A novel non-viral vector LNT-PEI-FA was successfully synthesized and characterized, which may be applied in gene transfection research in the future.
目的
开发一种新型基因递送载体，该载体由接枝到天然多糖上并与叶酸偶联的低分子量聚乙烯亚胺（LNT-PEI-FA）组成。
方法
分别通过¹H-NMR、FT-IR和TGA对LNT-PEI-FA的性质进行表征。测定LNT-PEI-FA/DNA复合物的粒径。通过凝胶电泳阻滞试验检测LNT-PEI-FA的DNA结合能力。
结果
LNT-PEI-FA/DNA复合物的粒径约为200nm。凝胶电泳显示，当N/P比为1.8（W/W）时，该聚合物能够完全凝聚DNA。体外实验表明，其在A293和B16细胞系中具有高效的基因转染能力。
结论
成功合成并表征了一种新型非病毒载体LNT-PEI-FA，其未来可能应用于基因转染研究。
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