阿司匹林-聚乙烯亚胺-β-环糊精作为一种新型的基因转移非病毒载体
[Aspirin-PEI-beta-CyD as a novel non-viral vector for gene transfer].
作者信息
Wang Zhong-Ren, Chen Dan, Zhou Jun, Tang Gu-Ping
机构信息
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):46-52. doi: 10.3785/j.issn.1008-9292.2009.01.007.
OBJECTIVE
To develop a novel non-viral gene delivery vector based on PEI-beta-CyD as backbone modified with aspirin, and to identify its physicochemical characters.
METHODS
1, 1-carbonyldiimidazole (CDI) was used to bind aspirin onto PEI-beta-CyD to form PEI-beta-CyD-ASP. (1)H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16, Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 cells.
RESULT
The structure of PEI-beta-CyD-ASP was confirmed by (1)H-NMR, FT-IR, UV and XRD, which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells.
CONCLUSION
The synthesized aspirin-PEI-beta-CyD might be a potential gene delivery vector.
目的
构建一种以聚乙烯亚胺-β-环糊精（PEI-β-CyD）为骨架、经阿司匹林修饰的新型非病毒基因递送载体，并鉴定其理化性质。
方法
采用1,1-羰基二咪唑（CDI）将阿司匹林连接到PEI-β-CyD上，形成PEI-β-CyD-ASP。利用核磁共振氢谱（¹H-NMR）、傅里叶变换红外光谱（FT-IR）、紫外光谱（UV）和X射线衍射（XRD）对聚合物结构进行确认。通过凝胶阻滞试验证明其凝聚能力。采用MTT法检测该聚合物对B16、Hela和A293细胞系的细胞活力。在B16细胞中检测该聚合物的转染效率。
结果
¹H-NMR、FT-IR、UV和XRD证实了PEI-β-CyD-ASP的结构，其在N/P比为4时能有效凝聚质粒DNA。该共聚物在B16细胞中表现出低细胞毒性和高转染效率。
结论
合成的阿司匹林-PEI-β-CyD可能是一种潜在的基因递送载体。
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