Xia Chen, Chen Zhitao, Xia Zhining
College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.
Se Pu. 2008 Nov;26(6):677-81.
Using an achiral ionic liquid, 1-butyl-3-methylimidazolium chlorine ([ BMIM] Cl), as an additive and beta-cyclodextrin (beta-CD) as a chiral selector, the enantiomers of chlorpheniramine, the precursor of chloramphenicol and of loxacin were separated by capillary zone electrophoresis. This work was directed to the study of the association of [BMIM] Cl to the chiral selector beta-CD and the possible effects of [BMIM] Cl on chiral separation. Simultaneously, the separation performances were studied when only containing beta-CD in the buffer. The results showed that there are synergistic effects of [BMIM] Cl as an additive for the enantiomeric separations. [BMIM] Cl can not only remarkably increase the separation selectivity and resolution of the enantiomers, but also effectively restrain the adsorption of the sample molecules and improve the peak shape. [BMIM] Cl as an additive of chiral separation can provide a new method for the separation of chiral drugs which are hard separable under common electrophoresis conditions.
使用非手性离子液体1-丁基-3-甲基咪唑氯盐（[BMIM]Cl）作为添加剂，β-环糊精（β-CD）作为手性选择剂，通过毛细管区带电泳分离了氯苯那敏、氯霉素前体和洛美沙星的对映体。这项工作旨在研究[BMIM]Cl与手性选择剂β-CD的缔合以及[BMIM]Cl对手性分离的可能影响。同时，研究了缓冲液中仅含有β-CD时的分离性能。结果表明，[BMIM]Cl作为对映体分离的添加剂具有协同作用。[BMIM]Cl不仅能显著提高对映体的分离选择性和分辨率，还能有效抑制样品分子的吸附并改善峰形。[BMIM]Cl作为手性分离添加剂可为在普通电泳条件下难以分离的手性药物的分离提供一种新方法。
