Chen Lanzhen, Li Guifen, Xue Xiaofeng, Wu Liming, Zhao Jing, Huang Jingping, Yuan Hancheng
Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Se Pu. 2008 Nov;26(6):736-9.
Several different extraction procedures including perchloric acid extraction, boiling water extraction and boiling magnesium sulfate solution extraction were studied for the extraction of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) from the royal jelly. Among these methods, the extraction with 5% perchloric acid at below 4 degrees C was the optimum extraction method. A simple, fast and sensitive ultra-performance liquid chromatographic (UPLC) method was developed for the determination of ATP, ADP and AMP in royal jelly. The separation was achieved within 4 min using a BEH Shield RP18 column (100 mm x 2.1 mm, 1.7 microm) with 50 mmol/L monoammonium phosphate solution (pH 6.5) and acetonitrile as the mobile phase. The spiked recoveries of ATP, ADP and AMP were 84.1% -94.3%, 86.2% -93.7% and 91.0% -104.3%, respectively. The relative standard deviations were less than 10%. This method was successfully applied to the analysis of some royal jelly samples from beekeepers and markets for the investigation of distribution of ATP, ADP and AMP in royal jelly samples.
研究了几种不同的提取方法，包括高氯酸提取、沸水提取和硫酸镁溶液煮沸提取，用于从蜂王浆中提取三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和一磷酸腺苷（AMP）。在这些方法中，4℃以下用5%高氯酸提取是最佳提取方法。建立了一种简单、快速、灵敏的超高效液相色谱（UPLC）法测定蜂王浆中的ATP、ADP和AMP。使用BEH Shield RP18柱（100 mm×2.1 mm，1.7μm），以50 mmol/L磷酸二氢铵溶液（pH 6.5）和乙腈为流动相，4分钟内实现分离。ATP、ADP和AMP的加标回收率分别为84.1%-94.3%、86.2%-93.7%和91.0%-104.3%。相对标准偏差小于10%。该方法成功应用于分析一些来自养蜂人和市场的蜂王浆样品，以研究蜂王浆样品中ATP、ADP和AMP的分布情况。
