蜂王浆中腺苷磷酸、核苷和核碱基分布的鉴定。
Identification of the distribution of adenosine phosphates, nucleosides and nucleobases in royal jelly.
作者信息
Wu Liming, Chen Lanzhen, Selvaraj Jonathan Nimal, Wei Yue, Wang Yong, Li Yi, Zhao Jing, Xue Xiaofeng
机构信息
Apicultural Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
出版信息
Food Chem. 2015 Apr 15;173:1111-8. doi: 10.1016/j.foodchem.2014.10.137. Epub 2014 Nov 3.
Nucleotides, nucleosides and nucleobases play a greater role in the physiological activity of organisms which are highly present in royal jelly (RJ). The objective of the present study is to develop a HPLC method to simultaneous determine nucleotides, nucleosides and nucleobases in RJ and access them in fresh and commercial RJ samples. The LOD and LOQ were 12.2-99.6 μg/L and 40.8-289.4 μg/L, respectively with nearly 100.9% recoveries. Except uric acid, all other compounds were found in RJ samples. Significant difference in the average content of compounds in fresh (2682.93 mg/kg) and commercial samples (3152.78 mg/kg) were observed. AMP, adenosine and adenine were found predominant in all the samples. Significant higher levels of ATP, ADP and AMP was seen in fresh RJ samples, and IMP, uridine, guanosine, and thymidine was seen in commercial RJ samples. The investigated compounds can be used as indexes for assessment RJ freshness and quality.
核苷酸、核苷和核碱基在蜂王浆（RJ）中大量存在，它们在生物体的生理活动中发挥着更重要的作用。本研究的目的是开发一种高效液相色谱法，用于同时测定RJ中的核苷酸、核苷和核碱基，并对新鲜和市售RJ样品中的这些成分进行分析。检测限和定量限分别为12.2 - 99.6μg/L和40.8 - 289.4μg/L，回收率接近100.9%。除尿酸外，在RJ样品中发现了所有其他化合物。观察到新鲜样品（2682.93mg/kg）和市售样品（3152.78mg/kg）中化合物的平均含量存在显著差异。在所有样品中，AMP、腺苷和腺嘌呤含量最高。新鲜RJ样品中ATP、ADP和AMP的含量显著较高，而市售RJ样品中IMP、尿苷、鸟苷和胸苷的含量较高。所研究的化合物可作为评估RJ新鲜度和质量的指标。
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