[碱性成纤维细胞生长因子对成骨细胞膜上整合素α2、α5、β1 mRNA表达的调控]
[Regulation of integrin alpha2, alpha5, beta1 mRNA expression on osteoblasts membrane by bFGF].
作者信息
Xia Lu, Wang Pei-zhi, Liang Xing, Xu Ling, Wang Guo-ping
机构信息
Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Nov;39(6):909-11.
OBJECTIVE
To observe the regulation of bFGF on mRNA expression of integrin subunits alpha2, alpha5, beta1, in osteoblasts.
METHODS
Osteoblasts were pre-cultured for 24 h with F12 medium containing bFGF with different concentration and planted on the sand blasting titanium disks. Three days after planting, osteoblasts were harvested. The mRNA expression of integrin subunit alpha2, alpha5, beta1, were examined by immunofluorescent quantitative real-time polymerase chain reaction (RT-PCR).
RESULTS
The mRNA expression of integrin beta1 is most abundant and the mRNA expressions of integrin alpha2, as are relatively lower than integrin beta1. In the study, the mRNA expression levels of integrin alpha2, alpha5 and beta1 were up-regulated after bFGF stimulation. However, the effective concentration range of bFGF to the three subunits was different.
CONCLUSION
bFGF could up-regulate the mRNA expression of integrin subunits in osteoblasts.
目的
观察碱性成纤维细胞生长因子（bFGF）对成骨细胞中整合素α2、α5、β1亚基mRNA表达的调控作用。
方法
将成骨细胞用含不同浓度bFGF的F12培养基预培养24小时，然后接种于喷砂钛盘上。接种三天后，收获成骨细胞。采用免疫荧光定量实时聚合酶链反应（RT-PCR）检测整合素α2、α5、β1亚基的mRNA表达。
结果
整合素β1的mRNA表达最为丰富，整合素α2的mRNA表达相对低于整合素β1。在本研究中，bFGF刺激后整合素α2、α5和β1的mRNA表达水平上调。然而，bFGF对这三个亚基的有效浓度范围不同。
结论
bFGF可上调成骨细胞中整合素亚基的mRNA表达。
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