[缺氧条件下体外培养的血管内皮细胞中水通道蛋白1的表达]
[AQP1 expression in vascular endothelial cells in vitro under hypoxia conditions].
作者信息
Wu Qin-qin, Chen Yu-cheng, Jiang Xiao-fei, Zeng Zhi
机构信息
Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Nov;39(6):916-20.
OBJECTIVE
To investigate the expression of AQP1 in endothelial cells under hypoxia conditions in vitro.
METHODS
The cultured human umbilical vein endothelial cells were randomly assigned to hypoxia groups (exposed to 1% of O2 for 3 h, 6 h, 12 h and 24 h respectively), and control group (ordinary culture). The expressions of AQP1 mRNA and protein were detected by real time PCR and immunohistochemistry respectively.
RESULTS
The expressions of AQP1 protein and mRNA increased after exposure to hypoxia initially and then decreased afterwards.
CONCLUSION
Hypoxia regulates the expression of AQP1 in vascular endothelial cells.
目的
研究体外缺氧条件下血管内皮细胞中水通道蛋白1（AQP1）的表达情况。
方法
将培养的人脐静脉内皮细胞随机分为缺氧组（分别暴露于1%氧气环境3小时、6小时、12小时和24小时）和对照组（常规培养）。分别采用实时荧光定量PCR和免疫组织化学法检测AQP1 mRNA和蛋白的表达。
结果
缺氧处理后，AQP1蛋白和mRNA的表达先升高后降低。
结论
缺氧可调节血管内皮细胞中AQP1的表达。
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