[铁调素对大鼠酒精性肝病发展的影响]

[Effect of hepcidin on the development of alcoholic liver disease in rats].

作者信息

Xu Li, Chen Dao-bang, Jiang Nan, Zhao Yuan, Shi Kai, Deng Yihao

机构信息

Department of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Nov;39(6):936-9.

PMID:19253830

Abstract

OBJECTIVE

To establish an alcoholic liver disease model in rats to study the effect of Hepcidin on the development of alcoholic liver disease.

METHODS

Fourty SD rats were randomly divided into 2 groups, 20 for each group. Saline [10 mL/(kg x d)] and 56% ethano infusion [10 mL/(kg x d)] were given orally to the rats for 12 weeks for the control and experimental group, respectively. The serum ALT, AST, and iron, and the superoxide dismutase (SOD) activity and malonaldehyde (MDA) content in the hepatic tissue were determined. RT-PCR was employed to examine the expression of Hepcidin mRNA in the liver. The histopathological changes of the liver tissues were examined through HE, Sudan IV and immunohistochemical stainings.

RESULTS

The rats in the experimental group had greater serum ALT, AST and iron, and decreased SOD activity and increased MDA activity than those in the control group (P<0.01). The rats in the experimental group had enlarged livers, with rough surface and grey-yellow color. The rats in the experimental group had more local hepatocellular necrosis, inflammatory cells, and large fat droplets, and larger red tangerine staining sectors in the livers than those in the control group (P<0.05). Higher positive expressions of TNF-alpha and IL-6 in the liver tissues were found in the experimental group than in the control group (P<0.01). The rats in the experimental group had lower levels of mRNA of Hepcidin than those in the control group (P<0.01).

CONCLUSION

The rats with alcoholic livers have increased expression of TNF-alpha and IL-6 and decreased expression of Hepcidin in the liver tissues.

摘要

目的

建立大鼠酒精性肝病模型，研究铁调素对酒精性肝病发展的影响。

方法

将40只SD大鼠随机分为2组，每组20只。对照组和实验组大鼠分别口服生理盐水[10 mL/(kg·d)]和56%乙醇溶液[10 mL/(kg·d)]，持续12周。测定血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、铁含量，以及肝组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。采用逆转录-聚合酶链反应（RT-PCR）检测肝脏中铁调素mRNA的表达。通过苏木精-伊红（HE）染色、苏丹IV染色和免疫组化染色检查肝组织的组织病理学变化。

结果

实验组大鼠血清ALT、AST和铁含量高于对照组，SOD活性降低，MDA活性升高（P<0.01）。实验组大鼠肝脏肿大，表面粗糙，颜色灰黄。实验组大鼠肝脏局部肝细胞坏死、炎症细胞和大脂滴更多，肝脏中红色橘黄色染色区域比对照组大（P<0.05）。实验组肝组织中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的阳性表达高于对照组（P<0.01）。实验组大鼠铁调素mRNA水平低于对照组（P<0.01）。