Laaksonen Päivi, Kivioja Jani, Paananen Arja, Kainlauri Markku, Kontturi Kyösti, Ahopelto Jouni, Linder Markus B
VTT Biotechnology, VTT Technical Research Centre of Finland, FI-02044 VTT Espoo, Finland.
Langmuir. 2009 May 5;25(9):5185-92. doi: 10.1021/la804001e.
We present an approach where biomolecular self-assembly is used in combination with lithography to produce patterns of metallic nanoparticles on a silicon substrate. This is achieved through a two-step method, resulting in attachment of nanoparticles on desired sites on the sample surfaces, which allowed a detailed characterization. First, a genetically modified hydrophobin protein, NCysHFBI, was attached by self-assembly on a hydrophobic surface or a surface patterned with hydrophobic and hydrophilic domains. The next step was to label the protein layers with 17.8 nm gold nanoparticles, to allow microscopic characterization of the films. Kinetics and extent of attachment of nanoparticles were characterized by UV-vis spectroscopy and transmission electron microscopy. It was shown that the attachment of citrate-stabilized gold nanoparticles was strongly dependent on the electrostatic properties of the capping ligand layer and the density of nanoparticles in the monolayer could be controlled via pH. The resulting nanoparticle assemblies followed the original pattern created by optical lithography in high accuracy. We demonstrate that combining bottom-up and top-down nanotechnological approaches in a good balance can provide very effective ways to produce nanoscale components providing a functional interface between electronics and the biological world.
我们提出了一种方法，即利用生物分子自组装与光刻技术相结合，在硅基衬底上制备金属纳米颗粒图案。这是通过两步法实现的，使得纳米颗粒附着在样品表面的所需位置，从而能够进行详细表征。首先，通过自组装将一种基因改造的疏水蛋白NCysHFBI附着在疏水表面或具有疏水和亲水区域图案的表面上。下一步是用17.8纳米的金纳米颗粒标记蛋白质层，以便对薄膜进行显微镜表征。通过紫外可见光谱和透射电子显微镜对纳米颗粒附着的动力学和程度进行了表征。结果表明，柠檬酸盐稳定的金纳米颗粒的附着强烈依赖于封端配体层的静电性质，并且单层中纳米颗粒的密度可以通过pH值进行控制。所得的纳米颗粒组件能够高精度地遵循由光刻技术创建的原始图案。我们证明，在良好平衡的情况下结合自下而上和自上而下的纳米技术方法，可以提供非常有效的方式来生产纳米级组件，从而在电子学和生物世界之间提供一个功能界面。
