Berger Bryan W, Sallada Nathanael D
1Department of Biomedical Engineering, University of Virginia, Thornton Hall, P.O. Box 400259, Charlottesville, VA 22904 USA.
2Department of Chemical Engineering, University of Virginia, 214 Chem. Eng., 102 Engineers' Way, Charlottesville, VA 22904 USA.
J Biol Eng. 2019 Jan 23;13:10. doi: 10.1186/s13036-018-0136-1. eCollection 2019.
Hydrophobins are highly surface-active proteins that have versatile potential as agents for interface engineering. Due to the large and growing number of unique hydrophobin sequences identified, there is growing potential to engineer variants for particular applications using protein engineering and other approaches. Recent applications and advancements in hydrophobin technologies and production strategies are reviewed. The application space of hydrophobins is large and growing, including hydrophobic drug solubilization and delivery, protein purification tags, tools for protein and cell immobilization, antimicrobial coatings, biosensors, biomineralization templates and emulsifying agents. While there is significant promise for their use in a wide range of applications, developing new production strategies is a key need to improve on low recombinant yields to enable their use in broader applications; further optimization of expression systems and yields remains a challenge in order to use designed hydrophobin in commercial applications.
疏水蛋白是具有高度表面活性的蛋白质，作为界面工程试剂具有多种潜在用途。由于已鉴定出的独特疏水蛋白序列数量众多且不断增加，利用蛋白质工程和其他方法为特定应用设计变体的潜力也在不断增长。本文综述了疏水蛋白技术和生产策略的最新应用与进展。疏水蛋白的应用领域广泛且不断扩大，包括疏水性药物的增溶与递送、蛋白质纯化标签、蛋白质和细胞固定化工具、抗菌涂层、生物传感器、生物矿化模板以及乳化剂。尽管它们在广泛应用中具有巨大潜力，但开发新的生产策略是提高低重组产量以使其能在更广泛应用中使用的关键需求；为了将设计的疏水蛋白用于商业应用，进一步优化表达系统和产量仍然是一项挑战。
