外阴前庭炎综合征女性中炎性小体成分NALP3编码基因的多态性与复发性念珠菌性外阴阴道炎

Polymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome.

作者信息

Lev-Sagie Ahinoam, Prus Diana, Linhares Iara M, Lavy Yuval, Ledger William J, Witkin Steven S

机构信息

Division of Immunology and Infectious Diseases, Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY 10065, USA.

出版信息

Am J Obstet Gynecol. 2009 Mar;200(3):303.e1-6. doi: 10.1016/j.ajog.2008.10.039.

DOI:10.1016/j.ajog.2008.10.039

PMID:19254587

Abstract

OBJECTIVE

Patients with vulvar vestibulitis syndrome (VVS) and control subjects were tested for a polymorphism in the gene coding for the NALP3 component of inflammasomes, cytoplasmic structures regulating interleukin (IL)-1beta production.

STUDY DESIGN

DNA from 143 women with VVS and 182 control women were tested for a length polymorphism in intron 4 of the gene (CIAS1) that codes for NALP3. Vestibular tissue was examined for NALP3 expression. Whole blood cultures were tested for Candida albicans-induced IL-1beta production.

RESULTS

The allele 12 frequency was higher in control subjects than in the patients with VVS (P = .02). Among patients with VVS and a self-reported history of recurrent vulvovaginal candidiasis (RVVC), the allele 7 frequency was 43.9% as compared with 30.8% in patients with no history of RVVC and 26.9% in control women (P = .035 vs other patients and .001 vs control subjects). NALP3 was identified in vestibular tissue. C albicans-induced IL-1beta production was reduced in samples from women with the 7,7 genotype (P = .030).

CONCLUSION

Polymorphism in the CIAS1 gene may play a central role in the triggering of VVS in a subset of patients.

摘要

目的

对患有外阴前庭炎综合征（VVS）的患者和对照受试者进行检测，以确定编码炎性小体（调节白细胞介素（IL）-1β产生的细胞质结构）NALP3成分的基因中的多态性。

研究设计

对143名患有VVS的女性和182名对照女性的DNA进行检测，以确定编码NALP3的基因（CIAS1）第4内含子中的长度多态性。检查前庭组织中的NALP3表达。对全血培养物进行白色念珠菌诱导的IL-1β产生检测。

结果

对照受试者的等位基因12频率高于患有VVS的患者（P = 0.02）。在有复发性外阴阴道念珠菌病（RVVC）自我报告病史的VVS患者中，等位基因7频率为43.9%，无RVVC病史的患者中为30.8%，对照女性中为26.9%（与其他患者相比P = 0.035，与对照受试者相比P = 0.001）。在前庭组织中鉴定出NALP3。7,7基因型女性样本中白色念珠菌诱导的IL-1β产生减少（P = 0.030）。