Chou C L, Guy R D, Uthe J F
Marine Chemistry Division, Scotia-Fundy Region, Department of Fisheries and Oceans, Halifax, N.S., Canada.
Sci Total Environ. 1991 Jun;105:61-71. doi: 10.1016/0048-9697(91)90329-d.
The reactivity of EDTA, Cu2+, and copper citrate with two metallothionein preparations (MT-1 and MT-2) isolated from the digestive gland of Cd-contaminated American lobster (Homarus americanus) was studied. Under pseudo-first-order conditions, metallothioneins reacted with EDTA for removal of Cd2+ in a multiphasic manner. Cadmium(+II) removal by Cu2+ was complex and non-stoichiometric, suggesting different binding sites. Rabbit liver metallothionein reacted similarly. Cadmium removal from lobster metallothionein by copper citrate was slow and triphasic in nature. EDTA removed Cu2+ from lobster metallothionein very slowly.
研究了乙二胺四乙酸（EDTA）、铜离子（Cu2+）和柠檬酸铜与从受镉污染的美洲龙虾（美洲螯龙虾）消化腺中分离出的两种金属硫蛋白制剂（MT-1和MT-2）的反应活性。在准一级条件下，金属硫蛋白与EDTA以多相方式反应以去除镉离子（Cd2+）。铜离子去除镉（+II）的过程复杂且非化学计量的，表明存在不同的结合位点。兔肝金属硫蛋白的反应情况类似。柠檬酸铜从龙虾金属硫蛋白中去除镉的过程缓慢且呈三相性质。EDTA从龙虾金属硫蛋白中去除铜离子的速度非常慢。
