Mukai Yohei, Nakamura Teruya, Yoshioka Yasuo, Tsunoda Shin-ichi, Kamada Haruhiko, Nakagawa Shinsaku, Yamagata Yuriko, Tsutsumi Yasuo
Osaka University, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Mar 1;65(Pt 3):295-8. doi: 10.1107/S1744309109004461. Epub 2009 Feb 26.
Tumour necrosis factor receptor type 2 (TNFR2, TNFRSF1B) is an essential receptor for various host-defence functions of tumour necrosis factor alpha (TNF). As part of studies to determine the structure of TNFR2, the formation, crystallization and preliminary X-ray diffraction analysis of the TNF-TNFR2 complex are described. The TNF-TNFR2 complex, which comprises one TNF trimer and three TNFR2 monomers, was confirmed and purified by size-exclusion chromatography. Crystals of the TNF-TNFR2 complex were obtained using polyethylene glycol 3350 as a precipitant. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 74.5, b = 117.4, c = 246.8 A. Assuming the presence of two TNF-TNFR2 complexes in the asymmetric unit, the Matthews coefficient V(M) was 2.49 A(3) Da(-1) and the solvent content of the crystal was 50.7%. The crystal diffracted to 2.95 A resolution.
肿瘤坏死因子受体2型（TNFR2，TNFRSF1B）是肿瘤坏死因子α（TNF）各种宿主防御功能的重要受体。作为确定TNFR2结构研究的一部分，本文描述了TNF-TNFR2复合物的形成、结晶及初步X射线衍射分析。由一个TNF三聚体和三个TNFR2单体组成的TNF-TNFR2复合物通过尺寸排阻色谱法得到确认和纯化。以聚乙二醇3350作为沉淀剂获得了TNF-TNFR2复合物的晶体。该晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 74.5、b = 117.4、c = 246.8 Å。假设不对称单元中存在两个TNF-TNFR2复合物，马修斯系数V(M)为2.49 Å(3) Da(-1)，晶体的溶剂含量为50.7%。该晶体的衍射分辨率达到2.95 Å。
