Montell D J, Keshishian H, Spradling A C
Carnegie Institution of Washington, Baltimore, MD 21210.
Science. 1991 Oct 11;254(5029):290-3. doi: 10.1126/science.254.5029.290.
Somatic and germline cells interact during oogenesis to establish the pattern axes of the Drosophila eggshell and embryo. The role of the oocyte nucleus in pattern formation was tested with the use of laser ablation. Ablation in stage 6 to 9 egg chambers caused partial or complete ventralization of the eggshell, phenotypes similar to those of eggs produced by gurken or torpedo females. Accumulation of vasa protein at the posterior pole of treated oocytes was also disrupted. Thus the oocyte nucleus is required as late as stage 9 for dorsoventral patterning within the follicle cells and for polar plasm assembly in the oocyte.
在卵子发生过程中，体细胞和生殖细胞相互作用，以建立果蝇卵壳和胚胎的模式轴。利用激光消融技术测试了卵母细胞核在模式形成中的作用。在6至9期卵室中进行消融会导致卵壳部分或完全腹侧化，其表型与由gurken或torpedo雌性产生的卵相似。处理过的卵母细胞后极处vasa蛋白的积累也受到干扰。因此，卵母细胞核在卵泡细胞内的背腹模式形成以及卵母细胞中的极质组装中，直到9期都是必需的。
