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[哥斯达黎加结晶蔗糖对某些医学重要微生物的微生物污染及抗菌活性]

[Microbiological contamination and antimicrobial activity of cristalised cane sugar on some medically important microorganisms in Costa Rica].

作者信息

Pujol Verónica, Diaz Jendry, Rodríguez Evelyn, Arias María Laura

机构信息

Facultad de Microbiología y Centro de Investigación en Enfermedades Tropicales (CIET), Universidad de Costa Rica, 2060 San José, Costa Rica.

出版信息

Rev Biol Trop. 2008 Jun;56(2):431-7.

PMID:19256417
Abstract

Microbiological contamination and antimicrobial activity of cristalised cane sugar on some medically important microorganisms in Costa Rica. Unrefined cristalised cane sugar, obtained after the filtration and evaporation of sugar cane juice, is a nutritional product of traditional consumption in Costa Rica and other Neotropical countries. It has been used in the topic treatment of infected wounds, with satisfactory results even with some antibiotic-ressistant bacteria. We studied the microbiological quality of 50 commercial samples. The analyses included total aerobic and anaerobic bacteria plate count; aerobic and anaerobic spore count; mold and yeast count; total and fecal coliforms; and presence of Clostridium botulinum. The antimicrobial effect was tested for Staphylococcus aureus (ATCC 25923), S. epidermidis (UCR 2902), Pseudomonas aeruginosa (ATCC 9027), Escherichia coli (ATCC 25922), Salmonella enteritidis (ATCC 13076), Listeria monocytogenes (ATCC 19116) and Aspergillus niger (Asni 06). Most of the samples (76%) presented counts lower than 100 CFU/g especially for sporulated forms (90% lower than 20 CFU/g), the mold and yeast count was higher (38% higher than 10(2) CFU/g), demonstrating the importance of these microorganisms in the spoilage of the product; 76% of the samples presented fecal contamination; C. botulinum was not isolated with the methodology employed. No inhibitory effect was observed for A. niger, but all samples han an inhibitory effect over the other species, especially for P. aeruginosa and S. aureus.

摘要

哥斯达黎加结晶蔗糖对一些医学重要微生物的微生物污染及抗菌活性。未精制的结晶蔗糖是甘蔗汁经过过滤和蒸发后得到的,是哥斯达黎加和其他新热带国家传统消费的营养产品。它已被用于感染伤口的局部治疗,即使对一些耐抗生素细菌也取得了满意的效果。我们研究了50个商业样品的微生物质量。分析包括需氧菌和厌氧菌总数平板计数;需氧和厌氧芽孢计数;霉菌和酵母菌计数;总大肠菌群和粪大肠菌群;以及肉毒梭菌的存在情况。对金黄色葡萄球菌(ATCC 25923)、表皮葡萄球菌(UCR 2902)、铜绿假单胞菌(ATCC 9027)、大肠埃希菌(ATCC 25922)、肠炎沙门氏菌(ATCC 13076)、单核细胞增生李斯特菌(ATCC 19116)和黑曲霉(Asni 06)进行了抗菌效果测试。大多数样品(76%)的计数低于100 CFU/g,尤其是芽孢形成形式(90%低于20 CFU/g),霉菌和酵母菌计数较高(38%高于10² CFU/g),表明这些微生物在产品变质中的重要性;76%的样品存在粪便污染;采用所使用的方法未分离出肉毒梭菌。对黑曲霉未观察到抑制作用,但所有样品对其他菌种均有抑制作用,尤其是对铜绿假单胞菌和金黄色葡萄球菌。

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