Chimeddorj Battogtokh, Pak Chin Young, Damdin Avirmed, Okamoto Naoyuki, Miyagi Yohei
Department of Microbiology and Immunology, Health Sciences University of Mongolia, Mongolia.
Asian Pac J Cancer Prev. 2008 Oct-Dec;9(4):563-8.
Infection with high-risk human papillomavirus (HPV) is a critical factor associated with carcinogenesis of the uterine cervix. HPV-16 is most frequently found, and is further subclassified into intratypic variants based on the nucleotide sequences of the viral genes. Although certain HPV-16 variants are reported to be associated with the progression of cervical lesions, these relationships remain controversial with different results for different populations. To provide data for another population, we investigated the prevalence of HPV-16 and distributions of its intratypic variants among Mongolian women with cervical intraepithelial neoplasia (CIN) and invasive cervical cancer.
We analyzed samples from 374 randomly selected women who attended the National Cancer Center of Mongolia between January 2002 and July 2007, including 147 invasive cervical cancer patients, 127 CIN patients and 100 age-matched controls who were cytologically normal. HPV genotyping was initially conducted, followed by variant analysis for HPV-16-positive samples by nucleotide sequencing of the E6 gene. The HPV data were evaluated statistically for correlations with the patients' clinical data.
HPV genotyping detected 101 HPV-16-positive samples. Among these samples, 92 were available for subsequent variant analysis, including 66 invasive cervical cancer samples, 25 CIN samples and 1 cytologically normal sample. A total of 14 different variants were identified. All 14 variants belonged to the European lineage, and the European prototype was detected in 66% (61/92) of the samples. Among the remaining 31 variants, variants with the T350G nucleotide change were predominant (13/31, 42%), followed by variants containing G94A (11/31, 35%), G176A (4/31, 13%) and G274T (2/31, 7%). There were no significant differences among all the variants regarding their distributions in CIN and invasive cervical cancers.
HPV-16 variants of the European lineage were exclusively distributed among the Mongolian women examined, and the European prototype was overwhelmingly predominant. Since no significant differences were found between the types of variants and severities of the cervical lesions, it is possible that racial or geographic factors may have some influences on these relationships.
高危型人乳头瘤病毒（HPV）感染是与子宫颈癌发生相关的关键因素。HPV-16最为常见，并根据病毒基因的核苷酸序列进一步分为不同的亚型变体。尽管据报道某些HPV-16变体与宫颈病变的进展相关，但这些关系仍存在争议，不同人群的结果各异。为另一人群提供数据，我们调查了蒙古宫颈上皮内瘤变（CIN）和浸润性宫颈癌女性中HPV-16的流行情况及其亚型变体的分布。
我们分析了2002年1月至2007年7月期间在蒙古国家癌症中心就诊的374名随机选择女性的样本，包括147例浸润性宫颈癌患者、127例CIN患者和100例细胞学正常的年龄匹配对照。首先进行HPV基因分型，然后通过E6基因的核苷酸测序对HPV-16阳性样本进行变体分析。对HPV数据与患者临床数据的相关性进行统计学评估。
HPV基因分型检测到101个HPV-16阳性样本。在这些样本中，92个可用于后续变体分析，包括66个浸润性宫颈癌样本、25个CIN样本和1个细胞学正常样本。共鉴定出14种不同的变体。所有14种变体均属于欧洲谱系，66%（61/92）的样本检测到欧洲原型。在其余31种变体中，T350G核苷酸变化的变体占主导（13/31，42%），其次是含有G94A（11/31，35%）、G176A（4/31，13%）和G274T（2/31，7%）的变体。所有变体在CIN和浸润性宫颈癌中的分布无显著差异。
欧洲谱系的HPV-16变体仅在接受检查的蒙古女性中分布，且欧洲原型占绝对优势。由于在变体类型与宫颈病变严重程度之间未发现显著差异，种族或地理因素可能对这些关系有一定影响。
